POFUT2 antibody (N-Term)

Catalog No. ABIN2782385

Product Details anti-POFUT2 Antibody

Target: POFUT2 (Protein O-Fucosyltransferase 2 (POFUT2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio), Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POFUT2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GAASRRRYLL YDVNPPEGFN LRRDVYIRIA SLLKTLLKTE EWVLVLPPWG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against POFUT2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human POFUT2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 429 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-POFUT2 antibody (ABIN2782385)

Luo, Koles, Vorndam, Haltiwanger, Panin: "Protein O-fucosyltransferase 2 adds O-fucose to thrombospondin type 1 repeats." in: The Journal of biological chemistry, Vol. 281, Issue 14, pp. 9393-9, (2006) (PubMed).

Target Details for POFUT2

Target: POFUT2 (Protein O-Fucosyltransferase 2 (POFUT2))

Alternative Name: POFUT2 (POFUT2 Products)

Synonyms: C21orf80 antibody, FUT13 antibody, fc46a11 antibody, wu:fc46a11 antibody, zgc:194822 antibody, 2310011G23Rik antibody, AI256847 antibody, BC003494 antibody, protein O-fucosyltransferase 2 antibody, POFUT2 antibody, pofut2 antibody, Pofut2 antibody

Background: POFUT2 catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in thrombospondin type 1 repeats. Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF, MIM 131530)-like repeats or thrombospondin (THBS, see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM].
Alias Symbols: C21orf80, FUT13
Protein Interaction Partner: USP22,
Protein Size: 429

Molecular Weight: 50 kDa

Gene ID: 23275

NCBI Accession: NM_133635, NP_598368

UniProt: Q9Y2G5