BAAT antibody (N-Term)

Catalog No. ABIN2782317

Product Details anti-BAAT Antibody

Target: BAAT (Bile Acid CoA: Amino Acid N-Acyltransferase (Glycine N-Choloyltransferase) (BAAT))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Horse, Rabbit, Cow, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAAT antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: IQLTATPVSA LVDEPVHIRA TGLIPFQMVS FQASLEDENG DMFYSQAHYR

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against BAAT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human BAAT

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 418 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-BAAT antibody (ABIN2782317)

Aleksunes, Yeager, Wen, Cui, Klaassen: "Repression of hepatobiliary transporters and differential regulation of classic and alternative bile acid pathways in mice during pregnancy." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 130, Issue 2, pp. 257-68, (2012) (PubMed).

Tougou, Fukuda, Ito, Yamazaki, Fujio, Azuma: "Genetic polymorphism of bile acid CoA: amino acid N-acyltransferase in Japanese individuals." in: Drug metabolism and pharmacokinetics, Vol. 22, Issue 2, pp. 125-8, (2007) (PubMed).

Target Details for BAAT

Target: BAAT (Bile Acid CoA: Amino Acid N-Acyltransferase (Glycine N-Choloyltransferase) (BAAT))

Alternative Name: BAAT (BAAT Products)

Synonyms: BACAT antibody, BAT antibody, AI118337 antibody, AI158864 antibody, kan-1 antibody, BAAT antibody, bile acid-CoA:amino acid N-acyltransferase antibody, Bile acid-CoA:amino acid N-acyltransferase antibody, bile acid-Coenzyme A: amino acid N-acyltransferase antibody, bile acid CoA:amino acid N-acyltransferase antibody, BAAT antibody, RPIC_RS10270 antibody, Bcav_2277 antibody, Rpic12D_1765 antibody, Baat antibody, LOC481635 antibody, LOC100054567 antibody, LOC786798 antibody

Background: BAAT is a liver enzyme that catalyzes the transfer of C24 bile acids from the acyl-CoA thioester to either glycine or taurine, the second step in the formation of bile acid-amino acid conjugates. The bile acid conjugates then act as a detergent in the gastrointestinal tract, which enhances lipid and fat-soluble vitamin absorption. Defects in this gene are a cause of familial hypercholanemia (FHCA). The protein encoded by this gene is a liver enzyme that catalyzes the transfer of C24 bile acids from the acyl-CoA thioester to either glycine or taurine, the second step in the formation of bile acid-amino acid conjugates. The bile acid conjugates then act as a detergent in the gastrointestinal tract, which enhances lipid and fat-soluble vitamin absorption. Defects in this gene are a cause of familial hypercholanemia (FHCA). Two transcript variants encoding the same protein have been found for this gene.
Alias Symbols: BACAT, BAT, FLJ20300, MGC104432
Protein Interaction Partner: GOLGA8F, GOLGA8EP, GOLGA8DP, PEX5, SLC7A11,
Protein Size: 418

Molecular Weight: 46 kDa

Gene ID: 570

NCBI Accession: NM_001701, NP_001692

UniProt: Q14032