NR1I3 antibody (Middle Region)
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- Target See all NR1I3 Antibodies
- NR1I3 (Nuclear Receptor Subfamily 1, Group I, Member 3 (NR1I3))
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Binding Specificity
- Middle Region
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Reactivity
- Human, Mouse, Rat, Dog, Guinea Pig, Cow, Pig, Horse, Rabbit, Zebrafish (Danio rerio)
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NR1I3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Sequence
- PVFRSLPIED QISLLKGAAV EICHIVLNTT FCLQTQNFLC GPLRYTIEDG
- Predicted Reactivity
- Cow: 100%, Dog: 100%, Guinea Pig: 92%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%
- Characteristics
- This is a rabbit polyclonal antibody against NR1I3. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the middle region of human NR1I3
- Top Product
- Discover our top product NR1I3 Primary Antibody
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 309 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- NR1I3 (Nuclear Receptor Subfamily 1, Group I, Member 3 (NR1I3))
- Alternative Name
- NR1I3 (NR1I3 Products)
- Synonyms
- AA209988 antibody, AI551208 antibody, CAR antibody, CAR-beta antibody, Care2 antibody, ESTM32 antibody, MB67 antibody, CAR1 antibody, CXR antibody, nuclear receptor subfamily 1 group I member 3 antibody, nuclear receptor subfamily 1, group I, member 3 antibody, NR1I3 antibody, Nr1i3 antibody
- Background
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NR1I3 mediates the induction of transcription of cytochrome P450 (CYP) genes by phenobarbital (PB) and PB-type inducers. NR1I3 activation induces hepatic expression of detoxification enzymes and transporters and increases liver size. NR1I3 can also regulate both liver homeostasis and tumorigenesis in response to xenobiotic stresses. This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. Multiple transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: CAR, CAR1, MB67, MGC150433, MGC97144, MGC97209
Protein Interaction Partner: HSP90AA1, RXRA, CHD9, SNX13, SNRPD3, IGH, PSMC5, PSMC4, EIF3I, SRC, MAP4, FTH1, HNF4A, NR0B2, PPARGC1A, MED1, NCOA1, POU1F1,
Protein Size: 309 - Molecular Weight
- 35 kDa
- Gene ID
- 9970
- NCBI Accession
- NM_001077471, NP_001070939
- UniProt
- Q6GZ85
- Pathways
- Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway
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