PIGA antibody (Middle Region)

Catalog No. ABIN2782189

Product Details anti-PIGA Antibody

Target: PIGA (Phosphatidylinositol Glycan Anchor Biosynthesis, Class A (PIGA))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Pig, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIGA antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: SVKSLCEGLE KAIFQLKSGT LPAPENIHNI VKTFYTWRNV AERTEKVYDR

Predicted Reactivity: Cow: 86%, Dog: 93%, Human: 100%, Mouse: 93%, Pig: 79%, Rabbit: 79%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against PIGA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PIGA

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 169 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PIGA antibody (ABIN2782189)

Traulsen, Pacheco, Dingli: "On the origin of multiple mutant clones in paroxysmal nocturnal hemoglobinuria." in: Stem cells (Dayton, Ohio), Vol. 25, Issue 12, pp. 3081-4, (2007) (PubMed).

Target Details for PIGA

Target: PIGA (Phosphatidylinositol Glycan Anchor Biosynthesis, Class A (PIGA))

Alternative Name: PIGA (PIGA Products)

Synonyms: GPI3 antibody, MCAHS2 antibody, PIG-A antibody, AI194334 antibody, Pig-a antibody, im:6911675 antibody, zgc:56589 antibody, phosphatidylinositol glycan anchor biosynthesis class A antibody, phosphatidylinositol glycan anchor biosynthesis, class A antibody, PIGA antibody, Piga antibody, piga antibody

Background: PIGA is a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene.This gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized.
Alias Symbols: GPI3, PIG-A
Protein Interaction Partner: UBC, ELAVL1, PIGY, DPM2, PIGQ, PIGH, PIGP,
Protein Size: 169

Molecular Weight: 19 kDa

Gene ID: 5277

NCBI Accession: NM_020473, NP_065206

UniProt: B3KUV7

Pathways: Inositol Metabolic Process