LCLAT1 antibody (Middle Region)

Catalog No. ABIN2781857

Product Details anti-LCLAT1 Antibody

Target: LCLAT1 (Lysocardiolipin Acyltransferase 1 (LCLAT1))

Binding Specificity: Middle Region

Reactivity: Mouse, Human, Rat, Rabbit, Cow, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LCLAT1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: YLYSLVKWYF IITIVIFVLQ ERIFGGLEII ELACYRLLHK QPHLNSKKNE

Predicted Reactivity: Cow: 93%, Dog: 92%, Guinea Pig: 92%, Horse: 93%, Human: 100%, Mouse: 92%, Rabbit: 100%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against LYCAT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human LYCAT

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 376 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-LCLAT1 antibody (ABIN2781857)

Zou, Synan, Li, Xiong, Manni, Liu, Chen, Zhao, Shiva, Tyurina, Jiang, Lee, Das, Ray, Ray, Kagan, Mallampalli: "LPS impairs oxygen utilization in epithelia by triggering degradation of the mitochondrial enzyme Alcat1." in: Journal of cell science, Vol. 129, Issue 1, pp. 51-64, (2016) (PubMed).

Target Details for LCLAT1

Target: LCLAT1 (Lysocardiolipin Acyltransferase 1 (LCLAT1))

Alternative Name: LYCAT (LCLAT1 Products)

Background: LYCAT is an acyl-CoA:lysocardiolipin acyltransferase. It possesses both lysophosphatidylinositol acyltransferase (LPIAT) and lysophosphatidylglycerol acyltransferase (LPGAT) activities. LYCAT recognizes both monolysocardiolipin and dilysocardiolipin as substrates with a preference for linoleoyl-CoA and oleoyl-CoA as acyl donors. LYCAT acts as a remodeling enzyme for cardiolipin, a major membrane polyglycerophospholipid. It converts lysophosphatidic acid (LPA) into phosphatidic acid (PA) with a relatively low activity. LYCAT is required for establishment of the hematopoietic and endothelial lineages.
Alias Symbols: ALCAT1, FLJ37965, UNQ1849, LYCAT, AGPAT8, 1AGPAT8, HSRG1849
Protein Interaction Partner: LNX1, GOPC, RNF2, UBC, ELAVL1,
Protein Size: 376

Molecular Weight: 44 kDa

Gene ID: 253558

NCBI Accession: NM_001002257, NP_001002257

UniProt: Q6UWP7