GLS2 antibody (Middle Region)

Catalog No. ABIN2781319

Product Details anti-GLS2 Antibody

Target: GLS2 (Glutaminase 2 (Liver, Mitochondrial) (GLS2))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Pig, Dog, Zebrafish (Danio rerio), Horse, Rabbit, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLS2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: FVGKEPSGLR YNKLSLNEEG IPHNPMVNAG AIVVSSLIKM DCNKAEKFDF

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against GLS2. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GLS2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 327 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GLS2 antibody (ABIN2781319)

Hu, Lausted, Yoo, Yan, Brightman, Chen, Wang, Bu, Hood: "Quantitative liver-specific protein fingerprint in blood: a signature for hepatotoxicity." in: Theranostics, Vol. 4, Issue 2, pp. 215-28, (2014) (PubMed).

Toriumi, Mouri, Narusawa, Aoyama, Ikawa, Lu, Nagai, Mamiya, Kim, Nabeshima: "Prenatal NMDA receptor antagonism impaired proliferation of neuronal progenitor, leading to fewer glutamatergic neurons in the prefrontal cortex." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 37, Issue 6, pp. 1387-96, (2012) (PubMed).

Target Details for GLS2

Target: GLS2 (Glutaminase 2 (Liver, Mitochondrial) (GLS2))

Alternative Name: GLS2 (GLS2 Products)

Synonyms: si:ch211-216k22.10 antibody, GA antibody, GLS antibody, LGA antibody, hLGA antibody, A330074B06Rik antibody, AI195532 antibody, Lga antibody, Ga antibody, glutaminase 2 antibody, glutaminase 2a (liver, mitochondrial) antibody, glutaminase 2 (liver, mitochondrial) antibody, glsA2 antibody, LOAG_03311 antibody, gls2a antibody, GLS2 antibody, Gls2 antibody

Background: GLS2 is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. This protein is functionally similar to the kidney glutaminase but is a little smaller in size. Originally thought to be liver-specific, this protein has been found in other tissues as well.
Alias Symbols: GA, GLS, LGA, MGC71567, hLGA
Protein Interaction Partner: SNTA1, GRHPR, TAX1BP3, PAG1, DLG2, INADL, DLG4, RGS3, DLG1, DLG3, CASK,
Protein Size: 327

Molecular Weight: 36 kDa

Gene ID: 27165

NCBI Accession: NM_013267

UniProt: Q9UI32

Pathways: Dicarboxylic Acid Transport, Warburg Effect