ECH1 antibody (N-Term)

Catalog No. ABIN2781318

Product Details anti-ECH1 Antibody

Target: ECH1 (Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ECH1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: PDHSYESLRV TSAQKHVLHV QLNRPNKRNA MNKVFWREMV ECFNKISRDA

Predicted Reactivity: Cow: 100%, Dog: 92%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 86%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ECH1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ECH1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 328 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ECH1 antibody (ABIN2781318)

Xie, Zhang, Lei, Ouyang, Du: "Ananas comosus L. Leaf Phenols and p-Coumaric Acid Regulate Liver Fat Metabolism by Upregulating CPT-1 Expression." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 903258, (2014) (PubMed).

Xie, Wang, Zhang, Li, Can, Qing, Kung, Zhang: "Enhanced peroxisomal ?-oxidation metabolism in visceral adipose tissues of high-fat diet-fed obesity-resistant C57BL/6 mice." in: Experimental and therapeutic medicine, Vol. 2, Issue 2, pp. 309-315, (2012) (PubMed).

Target Details for ECH1

Target: ECH1 (Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1))

Alternative Name: ECH1 (ECH1 Products)

Background: ECH1 is a member of the hydratase/isomerase superfamily. ECH1 shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The protein contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway.This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.
Alias Symbols: HPXEL
Protein Interaction Partner: FAM9B, HUWE1, TRAF1, UBC, MDM2, PPP6R3, IQCB1, STAT3, PEX5, EEF1D, DNAJB11, HNRNPUL1, OPA1, APP, HTT, EEF1G, HDAC5, ICT1, TLR10, PAFAH1B3, SERPINB9, CSTF2, TIMP1,
Protein Size: 328

Molecular Weight: 36 kDa

Gene ID: 1891

NCBI Accession: NM_001398, NP_001389

UniProt: Q13011

Pathways: Monocarboxylic Acid Catabolic Process