UBR1 antibody (N-Term)

Catalog No. ABIN2781277

Product Details anti-UBR1 Antibody

Target: UBR1 (Ubiquitin Protein Ligase E3 Component N-Recognin 1 (UBR1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBR1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: YKQLQKEYIS DDHDRSISIT ALSVQMFTVP TLARHLIEEQ NVISVITETL

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Yeast: 82%

Characteristics: This is a rabbit polyclonal antibody against UBR1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UBR1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1749 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-UBR1 antibody (ABIN2781277)

Yamaguchi, Moritani, Tanahashi, Osabe, Nomura, Fujita, Keshavarz, Kunika, Nakamura, Yoshikawa, Ichiishi, Shiota, Yasui, Inoue, Itakura: "Lack of association of genetic variation in chromosome region 15q14-22.1 with type 2 diabetes in a Japanese population." in: BMC medical genetics, Vol. 9, pp. 22, (2008) (PubMed).

Target Details for UBR1

Target: UBR1 (Ubiquitin Protein Ligase E3 Component N-Recognin 1 (UBR1))

Alternative Name: UBR1 (UBR1 Products)

Synonyms: Dmel\\CG9086 antibody, Ubr1 antibody, UBR1 antibody, JBS antibody, AI504731 antibody, RGD1562326 antibody, Ubr1 ubiquitin ligase antibody, ubiquitin protein ligase E3 component n-recognin 1 antibody, E3 ubiquitin-protein ligase ubr-1 antibody, Ubr1 antibody, ubr1 antibody, UBR1 antibody, ubr-1 antibody

Background: UBR1 is an E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. UBR1 may be involved in pancreatic homeostasis.The N-end rule pathway is one proteolytic pathway of the ubiquitin system. The recognition component of this pathway, encoded by this gene, binds to a destabilizing N-terminal residue of a substrate protein and participates in the formation of a substrate-linked multiubiquitin chain. This leads to the eventual degradation of the substrate protein. The protein described in this record has a RING-type zinc finger and a UBR-type zinc finger. Mutations in this gene have been associated with Johanson-Blizzard syndrome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: JBS, MGC142065, MGC142067
Protein Interaction Partner: CDC6, UBC, SHFM1, UBE2D1, CBX8, ZNF593, DNPH1, USP5, XPO1, PFAS, CSE1L, ATP6V1A, VCL, UCHL3, CUL2, RECQL4, TIRAP, DNAJB11, NUDT3, SPRY2, LAMTOR3, UBE2A, GSK3B, GADD45A, BCL2L1, UBE2Z, NSFL1C, UBXN1, UBXN7, FAF2, FAF1, FOS, HNRNPA1, NDUFA4L2, ARL15, BTBD2,
Protein Size: 1749

Molecular Weight: 200 kDa

Gene ID: 197131

NCBI Accession: NM_174916, NP_777576

UniProt: Q8IWV7