UBE3B antibody (Middle Region)

Catalog No. ABIN2781245

Product Details anti-UBE3B Antibody

Target: UBE3B (Ubiquitin Protein Ligase E3B (UBE3B))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE3B antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: VDEAGIDQDG VFKEFLEEII KRVFDPALNL FKTTSGDERL YPSPTSYIHE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against UBE3B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBE3B

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1068 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-UBE3B antibody (ABIN2781245)

Szafranski, Schindler, Taudien, Hiller, Huse, Jahn, Schreiber, Backofen, Platzer: "Violating the splicing rules: TG dinucleotides function as alternative 3' splice sites in U2-dependent introns." in: Genome biology, Vol. 8, Issue 8, pp. R154, (2008) (PubMed).

Target Details for UBE3B

Target: UBE3B (Ubiquitin Protein Ligase E3B (UBE3B))

Alternative Name: UBE3B (UBE3B Products)

Synonyms: si:dkey-189p24.3 antibody, BPIDS antibody, AI449831 antibody, AU020130 antibody, ubiquitin protein ligase E3B antibody, ubiquitin-protein ligase E3B antibody, ubiquitin protein ligase E3B L homeolog antibody, UBE3B antibody, ube3b antibody, LOC581811 antibody, ube3b.L antibody, Ube3b antibody

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE3B is a member of the E3 ubiquitin-conjugating enzyme family. UBE3B may interact with other proteins and play a role in stress response.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E3 ubiquitin-conjugating enzyme family. The encoded protein may interact with other proteins and play a role in stress response. Alternatively spliced transcript variants encoding the same protein isoform have been identified for this gene.
Alias Symbols: DKFZp586K2123, DKFZp686A1051, FLJ45294, MGC131858, MGC78388
Protein Interaction Partner: SHFM1, UBC, IQCB1,
Protein Size: 1068

Molecular Weight: 123 kDa

Gene ID: 89910

NCBI Accession: NM_130466, NP_569733

UniProt: Q7Z3V4