GRHL3 antibody (C-Term)

Catalog No. ABIN2781145

Product Details anti-GRHL3 Antibody

Target: GRHL3 (Grainyhead-Like 3 (GRHL3))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Dog, Rat, Rabbit, Cow, Guinea Pig, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRHL3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: EEVFDALMLK TPDLKGLRNA ISEKYGFPEE NIYKVYKKCK RGILVNMDNN

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 86%, Pig: 93%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against GRHL3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human GRHL3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 509 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GRHL3 antibody (ABIN2781145)

Darido, Georgy, Wilanowski, Dworkin, Auden, Zhao, Rank, Srivastava, Finlay, Papenfuss, Pandolfi, Pearson, Jane: "Targeting of the tumor suppressor GRHL3 by a miR-21-dependent proto-oncogenic network results in PTEN loss and tumorigenesis." in: Cancer cell, Vol. 20, Issue 5, pp. 635-48, (2011) (PubMed).

Target Details for GRHL3

Target: GRHL3 (Grainyhead-Like 3 (GRHL3))

Alternative Name: GRHL3 (GRHL3 Products)

Synonyms: GRHL3 antibody, SOM antibody, TFCP2L4 antibody, AI561912 antibody, Get1 antibody, Som antibody, Tfcp2l4 antibody, ct antibody, grainyhead like transcription factor 3 antibody, grainyhead-like 3 (Drosophila) antibody, grainyhead-like transcription factor 3 antibody, GRHL3 antibody, Grhl3 antibody

Background: GRHL3 is a member of the grainyhead family of transcription factors. GRHL3 interacts with leader-binding protein 32 (LBP-32) and brother of mammalian grainyhead (BOM), and may function as a transcription factor during development. Multiple transcript variants encoding distinct isoforms have been identified for this gene. This gene encodes a member of the grainyhead family of transcription factors. The encoded protein interacts with leader-binding protein 32 (LBP-32) and brother of mammalian grainyhead (BOM), and may function as a transcription factor during development. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Additional transcript variants have been described, but their biological nature has not been determined.
Alias Symbols: SOM, TFCP2L4
Protein Interaction Partner: PRMT6, PRMT5, PRMT1, GRHL1, GRHL3, GRHL2,
Protein Size: 509

Molecular Weight: 57 kDa

Gene ID: 57822

NCBI Accession: NM_198174, NP_937817