RRN3 antibody (Middle Region)

Catalog No. ABIN2781066

Product Details anti-RRN3 Antibody

Target: RRN3 (RRN3 RNA Polymerase I Transcription Factor (RRN3))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RRN3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: DPIYQVWEDM SAEELQEFKK PMKKDIVEDE DDDFLKGEVP QNDTVIGITP

Predicted Reactivity: Guinea Pig: 93%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against RRN3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RRN3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 651 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-RRN3 antibody (ABIN2781066)

Amit, Sela, Keren, Melamed, Muler, Shomron, Izraeli, Ast: "Biased exonization of transposed elements in duplicated genes: A lesson from the TIF-IA gene." in: BMC molecular biology, Vol. 8, pp. 109, (2008) (PubMed).

Target Details for RRN3

Target: RRN3 (RRN3 RNA Polymerase I Transcription Factor (RRN3))

Alternative Name: RRN3 (RRN3 Products)

Background: RRN3 is RNA polymerase I-specific transcription initiation factor. Phosphorylation of RRN3 by MAPK cascades links cell signaling with the control of gene expression, namely results in rRNA synthesis in turn cell growth.
Alias Symbols: DKFZp566E104, MGC104238, TIFIA, A-270G1.2
Protein Interaction Partner: UBC, ACTA1, Polr1e, POLR1B, EIF3L, NMI, RBM15, UBD, Trim28, ELAVL1, TAF1D, POLR1A, MYO1C, TAF1C, TAF1B, TAF1A, UBTF, TBP, PPP2R2A, CDK2, CCNE1, RIBIN, ACTB,
Protein Size: 651

Molecular Weight: 74 kDa

Gene ID: 54700

NCBI Accession: NM_018427, NP_060897

UniProt: Q9NYV6

Pathways: Negative Regulation of intrinsic apoptotic Signaling