HBP1 antibody (Middle Region)

Catalog No. ABIN2780873

Product Details anti-HBP1 Antibody

Target: HBP1 (HMG Box-Containing Protein 1 (HBP1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HBP1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: SVILGDRWKK MKNEERRMYT LEAKALAEEQ KRLNPDCWKR KRTNSGSQQH

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against HBP1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HBP1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 514 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HBP1 antibody (ABIN2780873)

Wang, Pan, Chen, Huang, Zhang: "The acetylation of transcription factor HBP1 by p300/CBP enhances p16INK4A expression." in: Nucleic acids research, Vol. 40, Issue 3, pp. 981-95, (2012) (PubMed).

Li, Bian, Liao, Li, Zhao: "miR-17-5p promotes human breast cancer cell migration and invasion through suppression of HBP1." in: Breast cancer research and treatment, Vol. 126, Issue 3, pp. 565-75, (2011) (PubMed).

Target Details for HBP1

Target: HBP1 (HMG Box-Containing Protein 1 (HBP1))

Alternative Name: HBP1 (HBP1 Products)

Synonyms: 1700058O05Rik antibody, BE629963 antibody, C330012F01Rik antibody, C86454 antibody, hbp1-B antibody, Hmgb1 antibody, wu:fj62c10 antibody, zgc:112297 antibody, high mobility group box transcription factor 1 antibody, HMG-box transcription factor 1 antibody, HMG-box transcription factor 1 L homeolog antibody, Hbp1 antibody, HBP1 antibody, hbp1.L antibody, hbp1 antibody

Background: HBP1 is a transcriptional repressor that binds to the promoter region of target genes. This protein plays a role in the regulation of the cell cycle and of the Wnt pathway. It binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter, HBP1 is enhanced by interaction with RB1. The protein also can disrupt the interaction between DNA and TCF4.
Alias Symbols: FLJ16340
Protein Interaction Partner: FBXW11, UBC, TMEM37, SMAD1, ZNF212, KIAA1279, EWSR1, CD2AP, EP300, CREBBP, MYC, ANKRD2, SIN3A, SAP30, TCF4, HDAC1, RBL2, RB1,
Protein Size: 514

Molecular Weight: 58 kDa

Gene ID: 26959

NCBI Accession: NM_012257, NP_036389

UniProt: O60381