TRIM32 antibody (N-Term)

Catalog No. ABIN2780865

Product Details anti-TRIM32 Antibody

Target: TRIM32 (Tripartite Motif Containing 32 (TRIM32))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM32 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MAAAAASHLN LDALREVLEC PICMESFTEE QLRPKLLHCG HTICRQCLEK

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 93%, Mouse: 93%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against TRIM32. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TRIM32

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 653 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TRIM32 antibody (ABIN2780865)

Streich, Ronchi, Connick, Haas: "Tripartite motif ligases catalyze polyubiquitin chain formation through a cooperative allosteric mechanism." in: The Journal of biological chemistry, Vol. 288, Issue 12, pp. 8209-21, (2013) (PubMed).

Kudryashova, Wu, Havton, Spencer: "Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component." in: Human molecular genetics, Vol. 18, Issue 7, pp. 1353-67, (2009) (PubMed).

Target Details for TRIM32

Target: TRIM32 (Tripartite Motif Containing 32 (TRIM32))

Alternative Name: TRIM32 (TRIM32 Products)

Background: TRIM32 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TRIM32 localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.
Alias Symbols: HT2A, BBS11, TATIP, LGMD2H
Protein Interaction Partner: PTPN11, PDE9A, PTCD2, CLIP4, KCTD9, UBQLN1, TRIM32, RABAC1, ABI2, SCGB1A1, UBE2E1, UBE2D1, UBC, TP53, SDCBP, UBE2U, SYT6, MYCN, CEP250, CUL3, GPRASP2, GLIS2, YWHAB, TP73, UBE2D3, UBE2D2, HSP90AA1, SFN, YWHAQ, YWHAZ, YWHAH, YWHAG, YWHAE, NOTCH1, UBD, TOP1,
Protein Size: 653

Molecular Weight: 72 kDa

Gene ID: 22954

NCBI Accession: NM_012210, NP_036342

UniProt: Q13049

Pathways: Negative Regulation of intrinsic apoptotic Signaling