NFYB antibody (N-Term)

Catalog No. ABIN2780750

Product Details anti-NFYB Antibody

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYB antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MTMDGDSSTT DASQLGISAD YIGGSHYVIQ PHDDTEDSMN DHEDTNGSKE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against NFYB. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NFYB

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 207 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NFYB antibody (ABIN2780750)

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, (2006) (PubMed).

Target Details for NFYB

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Alternative Name: NFYB (NFYB Products)

Synonyms: CBF-A antibody, CBF-B antibody, HAP3 antibody, NF-YB antibody, NFYB antibody, nfyb antibody, zgc:110533 antibody, zgc:110552 antibody, hap3 antibody, cbf-a antibody, cbf-b antibody, CBFB antibody, Nfya antibody, AA985999 antibody, Cbf-A antibody, nuclear transcription factor Y subunit beta antibody, nuclear transcription factor Y subunit beta L homeolog antibody, nuclear transcription factor Y, beta b antibody, nuclear transcription factor Y, beta a antibody, nuclear transcription factor-Y beta antibody, NFYB antibody, nfyb.L antibody, nfybb antibody, nfyba antibody, nfyb antibody, Nfyb antibody

Background: NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence, protein sequence alignments and experiments with mutants.The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence, protein sequence alignments and experiments with mutants.
Alias Symbols: HAP3, CBF-A, CBF-B, NF-YB
Protein Interaction Partner: AGTRAP, POLE4, DRAP1, NFYA, LYN, CDK2, CCNE1, CCNA2, TP53, SMYD3, NFYC, MAOB, UBC, CREBBP, SP1, HDAC1, EP300, KAT2B, TP73, TAF12, TAF11, TAF6, GRB2, YBX3, CEBPZ, C1QBP, TBP, CIITA, CNTN2, MYC, ELF1, YBX1,
Protein Size: 207

Molecular Weight: 23 kDa

Gene ID: 4801

NCBI Accession: NM_006166, NP_006157

UniProt: P25208

Pathways: Regulation of Lipid Metabolism by PPARalpha