XBP1 antibody (N-Term)

Catalog No. ABIN2780640

Product Details anti-XBP1 Antibody

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Binding Specificity: N-Term

Reactivity: Human, Cow, Rabbit, Pig, Horse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: LMVPAQRGAS PEAASGGLPQ ARKRQRLTHL SPEEKALRRK LKNRVAAQTA

Predicted Reactivity: Cow: 79%, Dog: 83%, Horse: 79%, Human: 100%, Pig: 93%, Rabbit: 92%

Characteristics: This is a rabbit polyclonal antibody against XBP1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human XBP1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 261 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for XBP1

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Alternative Name: XBP1 (XBP1 Products)

Background: XBP1 is a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. XBP1 is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator.This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.
Alias Symbols: TREB5, XBP2
Protein Interaction Partner: DERL1, UBE2I, HM13, XBP1, CREBZF, ATF6, ATF6B, UBC, SUMO2, PSMA8, PSMA6, PSMA5, ZNF440, PCBD2, ZNF580, TRIP4, H1FX, FUBP3, NR0B2, SSX4, SRSF1, RBL2, HDGF, H1F0, GLI1, IRE1, FOS, ESR1, FOXA1, POLR2A,
Protein Size: 261

Molecular Weight: 29 kDa

Gene ID: 7494

NCBI Accession: NM_005080, NP_005071

UniProt: P17861

Pathways: ER-Nucleus Signaling, Unfolded Protein Response