KLF4 antibody (C-Term)

Catalog No. ABIN2780577

Product Details anti-KLF4 Antibody

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Horse, Dog, Rabbit, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: AGCGKTYTKS SHLKAHLRTH TGEKPYHCDW DGCGWKFARS DELTRHYRKH

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against KLF4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human KLF4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 470 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-KLF4 antibody (ABIN2780577)

Natesampillai, Kerkvliet, Leung, Veldhuis: "Regulation of Kruppel-like factor 4, 9, and 13 genes and the steroidogenic genes LDLR, StAR, and CYP11A in ovarian granulosa cells." in: American journal of physiology. Endocrinology and metabolism, Vol. 294, Issue 2, pp. E385-91, (2008) (PubMed).

Target Details for KLF4

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Alternative Name: KLF4 (KLF4 Products)

Synonyms: EZF antibody, GKLF antibody, Gklf antibody, Zie antibody, KLF4 antibody, biklf antibody, biklf-A antibody, Kruppel like factor 4 antibody, Kruppel-like factor 4 (gut) antibody, Kruppel-like factor 4 antibody, Kruppel-like factor 4 (gut) L homeolog antibody, KLF4 antibody, Klf4 antibody, klf4 antibody, klf4.L antibody

Background: The mammalian Kruppel-like transcription factor, KLF4 is involved in preventing centrosome amplification following DNA damage caused by gamma-irradiation. It is both necessary and sufficient in preventing centrosome amplification following gamma-radiation-induced DNA damage and does so by transcriptionally suppressing cyclin E expression n. Kruppel-like factor 4 is also known to exhibit checkpoint function during the G1/S and G2/M transitions of the cell cycle.
Alias Symbols: EZF, GKLF
Protein Interaction Partner: EP300, CREBBP, SETD7, CTBP1, Dlg4, APP, CUL2, VHL, UBC, TCEB2, SP1, RELA, PRKCD, PPARG, SMARCA4, HDAC2, HDAC7, KAT5, HDAC1, HDAC5, ELK1, KLF6, TP53,
Protein Size: 470

Molecular Weight: 50 kDa

Gene ID: 9314

NCBI Accession: NM_004235, NP_004226

UniProt: O43474

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance