E2F2 antibody (Middle Region)

Catalog No. ABIN2780568

Product Details anti-E2F2 Antibody

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: DQFLSPTLAC SSPLISFSPS LDQDDYLWGL EAGEGISDLF DSYDLGDLLI

Predicted Reactivity: Cow: 92%, Dog: 100%, Guinea Pig: 91%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 75%

Characteristics: This is a rabbit polyclonal antibody against E2F2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human E2F2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 437 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for E2F2

Target: E2F2 (E2F Transcription Factor 2 (E2F2))

Alternative Name: E2F2 (E2F2 Products)

Synonyms: E2F-2 antibody, CG1071 antibody, Dmel\\CG1071 antibody, E2F antibody, E2F2 antibody, dE2F-2 antibody, dE2F2 antibody, dE2f2 antibody, de2f2 antibody, e2f2 antibody, 9230110J10 antibody, E130207A07 antibody, fb40b03 antibody, wu:fb40b03 antibody, E2F transcription factor 2 antibody, si:ch211-160f23.5 antibody, E2F2 antibody, E2f2 antibody, si:ch211-160f23.5 antibody

Background: E2F2 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46 % amino acid identity to E2F1.The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46 % amino acid identity to E2F1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: E2F-2
Protein Interaction Partner: RB1, GNB5, GIT2, TFDP1, RBL1, ARID3A, ELAVL1, ATAD2, KMT2D, KMT2A, RYBP, SETD8, BCAR1, TFDP2, BRD2, YY1, SP1, CDK3, RNF144A, FHL2, SPIB, RBL2, E2F4, MYBL2, EAPP, TP53BP2, PPP1R13B, TP53INP1, JMY, GAB2, CDC25A, CDC6, E2F1, UXT, MT1G,
Protein Size: 437

Molecular Weight: 47 kDa

Gene ID: 1870

NCBI Accession: NM_004091, NP_004082

UniProt: Q14209

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication