SUPT4H antibody (Middle Region)

Catalog No. ABIN2780482

Product Details anti-SUPT4H Antibody

Target: SUPT4H (SUPT4H1) (Suppressor of Ty 4 Homolog 1 (SUPT4H1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio), Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUPT4H antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: NCDAYLQMKG NREMVYDCTS SSFDGIIAMM SPEDSWVSKW QRVSNFKPGV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against SUPT4H1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SUPT4H1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 117 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SUPT4H

Target: SUPT4H (SUPT4H1) (Suppressor of Ty 4 Homolog 1 (SUPT4H1))

Alternative Name: SUPT4H1 (SUPT4H1 Products)

Background: SUPT4H1 is a component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
Alias Symbols: SPT4H, SUPT4H, SPT4
Protein Interaction Partner: UBC, tat, SUPT5H, ZMYND8, APP, LRIF1, HMP19, PDLIM1, EWSR1, XRCC5, HTATSF1, ANXA5,
Protein Size: 117

Molecular Weight: 13 kDa

Gene ID: 6827

NCBI Accession: NM_003168, NP_003159

UniProt: P63272