SPIB antibody (Middle Region)

Catalog No. ABIN2780475

Product Details anti-SPIB Antibody

Target: SPIB (Spi-B Transcription Factor (Spi-1/PU.1 Related) (SPIB))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPIB antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: WGQQKGNRKR MTYQKLARAL RNYAKTGEIR KVKRKLTYQF DSALLPAVRR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SPIB. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SPIB

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 262 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SPIB antibody (ABIN2780475)

Zhao, Yao, Xu, Lambeth, Smith, Kgosana, Wang, Nair: "A functional MicroRNA-155 ortholog encoded by the oncogenic Marek's disease virus." in: Journal of virology, Vol. 83, Issue 1, pp. 489-92, (2008) (PubMed).

Target Details for SPIB

Target: SPIB (Spi-B Transcription Factor (Spi-1/PU.1 Related) (SPIB))

Alternative Name: SPIB (SPIB Products)

Synonyms: spi-b antibody, spib antibody, spiba antibody, SPI-B antibody, B42b-like antibody, Spi-B antibody, Spi-B transcription factor L homeolog antibody, Spi-B transcription factor antibody, Spi-B transcription factor (Spi-1/PU.1 related) antibody, spib.L antibody, SPIB antibody, Spib antibody

Background: SPI1 (MIM 165170) and SPIB are members of a subfamily of ETS (see ETS1, MIM 164720) transcription factors. ETS proteins share a conserved ETS domain that mediates specific DNA binding. SPIB and SPI1 bind to a purine-rich sequence, the PU box (5-prime-GAGG
Alias Symbols: SPI-B
Protein Interaction Partner: TMEM37, SSB, GATA1, IRF4, MAPK3, MAPK8, CREBBP, TBP, CEBPB, RB1, SPI1, JUN, CSNK2A2, CSNK2A1, SPIB, E2F4, E2F3, E2F2, E2F1,
Protein Size: 262

Molecular Weight: 29 kDa

Gene ID: 6689

NCBI Accession: NM_003121, NP_003112

UniProt: Q01892