BTF3 antibody (Middle Region)

Catalog No. ABIN2780354

Product Details anti-BTF3 Antibody

Target: BTF3 (Basic Transcription Factor 3 (BTF3))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BTF3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: DSLTSLRRLA EALPKQSVDG KAPLATGEDD DDEVPDLVEN FDEASKNEAN

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 92%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against BTF3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human BTF3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 206 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-BTF3 antibody (ABIN2780354)

Thakur, Saxena, Singh, Haq, Katti, Singh, Tripathi: "Human beta casein fragment (54-59) modulates M. bovis BCG survival and basic transcription factor 3 (BTF3) expression in THP-1 cell line." in: PLoS ONE, Vol. 7, Issue 9, pp. e45905, (2012) (PubMed).

Target Details for BTF3

Target: BTF3 (Basic Transcription Factor 3 (BTF3))

Alternative Name: BTF3 (BTF3 Products)

Synonyms: BETA-NAC antibody, BTF3a antibody, BTF3b antibody, NACB antibody, BTF3 antibody, ci-btf3 antibody, 1700054E11Rik antibody, nacb antibody, btf3a antibody, btf3b antibody, beta-nac antibody, wu:fb05d06 antibody, zgc:153435 antibody, ATBTF3 antibody, F2H15.11 antibody, F2H15_11 antibody, basic transcription factor 3 antibody, basic transcription factor 3 antibody, basic transcription factor 3 S homeolog antibody, BTF3 antibody, ci-btf3 antibody, Btf3 antibody, btf3 antibody, btf3.S antibody

Background: BTF3 forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes.
Alias Symbols: NACB, BTF3a, BTF3b, BETA-NAC
Protein Interaction Partner: TXLNA, TXLNB, NACA, UBC, PDCD4, PPP2R1A, gag-pol, ESR1, CTNNB1, NACA2, RWDD4, PDRG1, RPL29, RPL11, APP, H2AFX, USP17L9P, POLR2B, MED21, CSNK2B,
Protein Size: 206

Molecular Weight: 23 kDa

Gene ID: 689

NCBI Accession: NM_001037637, NP_001032726

UniProt: P20290