TWIST1 antibody (C-Term)

Catalog No. ABIN2780347

Product Details anti-TWIST1 Antibody

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Horse, Zebrafish (Danio rerio), Pig, Dog, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TWIST1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: DKLSKIQTLK LAARYIDFLY QVLQSDELDS KMASCSYVAH ERLSYAFSVW

Predicted Reactivity: Cow: 100%, Dog: 79%, Guinea Pig: 79%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 79%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against TWIST1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TWIST1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 202 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TWIST1 antibody (ABIN2780347)

Guha, Bandyopadhyaya, Polumuri, Chumsri, Gade, Kalvakolanu, Ahmed et al.: "Nicotine promotes apoptosis resistance of breast cancer cells and enrichment of side population cells with cancer stem cell-like properties via a signaling cascade involving galectin-3, α9 nicotinic ..." in: Breast cancer research and treatment, Vol. 145, Issue 1, pp. 5-22, (2014) (PubMed).

Target Details for TWIST1

Target: TWIST1 (Twist Homolog 1 (Drosophila) (TWIST1))

Alternative Name: TWIST1 (TWIST1 Products)

Synonyms: scs antibody, Xtwi antibody, acs3 antibody, X-twi antibody, bpes2 antibody, bpes3 antibody, twist antibody, Xtwist antibody, MGC69439 antibody, twist1 antibody, ACS3 antibody, BPES2 antibody, BPES3 antibody, CRS1 antibody, SCS antibody, TWIST antibody, bHLHa38 antibody, AA960487 antibody, M-Twist antibody, Pde antibody, Ska10 antibody, Ska antibody, Twist antibody, pdt antibody, cTwist antibody, Twist1b antibody, twist-1 antibody, wu:fd04c01 antibody, zgc:101115 antibody, twist family bHLH transcription factor 1 antibody, twist family bHLH transcription factor 1 S homeolog antibody, twist basic helix-loop-helix transcription factor 1 antibody, twist homolog 1 (Drosophila) antibody, twist family bHLH transcription factor 1a antibody, TWIST1 antibody, twist1 antibody, twist1.S antibody, Twist1 antibody, twist1a antibody

Background: Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation.TWIST1 is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue, in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome.
Alias Symbols: SCS, ACS3, CRS1, BPES2, BPES3, TWIST, bHLHa38
Protein Interaction Partner: UBC, HOXA5, ETS2, TP53, KAT2B, SETD8, HES6, MTA2, BRAP, TCF3, RELA, PPP2CA, HDAC2, CHD3, TWIST2, ELSPBP1, WDR5, HDAC3, RBBP7, CHD4, SOX2, EP300, TWIST1, GLI3, MYOD1,
Protein Size: 202

Molecular Weight: 21 kDa

Gene ID: 7291

NCBI Accession: NM_000474, NP_000465

UniProt: Q15672

Pathways: p53 Signaling, Proton Transport, Tube Formation, Negative Regulation of Transporter Activity