RFX5 antibody (N-Term)
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- Target See all RFX5 Antibodies
- RFX5 (Regulatory Factor X 5 (RFX5))
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Binding Specificity
- N-Term
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Reactivity
- Human, Rat, Dog, Guinea Pig, Horse, Rabbit, Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RFX5 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- MAEDEPDAKS PKTGGRAPPG GAEAGEPTTL LQRLRGTISK AVQNKVEGIL
- Predicted Reactivity
- Dog: 86%, Guinea Pig: 79%, Horse: 86%, Human: 100%, Pig: 86%, Rabbit: 86%, Rat: 79%
- Characteristics
- This is a rabbit polyclonal antibody against RFX5. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Protein A purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the N terminal region of human RFX5
- Top Product
- Discover our top product RFX5 Primary Antibody
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 616 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- RFX5 (Regulatory Factor X 5 (RFX5))
- Alternative Name
- RFX5 (RFX5 Products)
- Synonyms
- regulatory factor X5 antibody, regulatory factor X, 5 (influences HLA class II expression) antibody, RFX5 antibody, Rfx5 antibody
- Background
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RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. RFX is a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX.A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS, MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX.A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS, MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined.
Protein Interaction Partner: UBC, MRPL53, SLIRP, SRPRB, CDV3, LSM7, RPS19, RBMS1, NDUFA7, NDUFA2, ANKRA2, RFXANK, MLLT1, HDAC4, CIITA, HDAC2, RFXAP,
Protein Size: 616 - Molecular Weight
- 68 kDa
- Gene ID
- 5993
- NCBI Accession
- NM_001025603, NP_001020774
- UniProt
- P48382
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