MITF antibody (Middle Region)

Catalog No. ABIN2780334

Product Details anti-MITF Antibody

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Cow, Rabbit, Sheep, Zebrafish (Danio rerio), Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MITF antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MGSKLEDILM DDTLSPVGVT DPLLSSVSPG ASKTSSRRSS MSMEETEHTC

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MITF. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MITF

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 419 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-MITF antibody (ABIN2780334)

Ursini, Cavalleri, Fazio, Angrisano, Iacovelli, Porcelli, Maddalena, Punzi, Mancini, Gelao, Romano, Masellis, Calabrese, Rampino, Taurisano, Di Giorgio, Keller, Tarantini, Sinibaldi, Quarto et al.: "BDNF rs6265 methylation and genotype interact on risk for schizophrenia. ..." in: Epigenetics, Vol. 11, Issue 1, pp. 11-23, (2016) (PubMed).

Target Details for MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Alternative Name: MITF (MITF Products)

Background: MITF is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
Alias Symbols: WS2A, MI, WS2, bHLHe32
Protein Interaction Partner: EBP, STUB1, HK3, EPAS1, LEF1, APP, HINT1, ELAVL1, CDK2, SUMO2, PSMD14, MARK3, PIAS3, PIAS2, PIAS1, SUMO1, UBE2I, PAX3, OTX2, PAX6, PATZ1, GSK3B, TFEC, EP300, RPS6KA1, TFEB, MAPK14, TFE3, MAPK1, SPI1, FOS, CTNNB1, CDKN1A, RB1,
Protein Size: 419

Molecular Weight: 47 kDa

Gene ID: 4286

NCBI Accession: NM_000248, NP_000239

Pathways: Chromatin Binding