ZNF265 antibody (N-Term)

Catalog No. ABIN2780239

Product Details anti-ZNF265 Antibody

Target: ZNF265 (Zranb2) (Zinc Finger Protein 265 (Zranb2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZNF265 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: RCGREKTTEA KMMKAGGTEI GKTLAEKSRG LFSANDWQCK TCSNVNWARR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against ZNF265. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ZNF265

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 330 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ZNF265 antibody (ABIN2780239)

Wang, Du, Li, Zhang: "Identification of the Zinc Finger Protein ZRANB2 as a Novel Maternal Lipopolysaccharide-binding Protein That Protects Embryos of Zebrafish against Gram-negative Bacterial Infections." in: The Journal of biological chemistry, Vol. 291, Issue 8, pp. 4019-34, (2016) (PubMed).

Target Details for ZNF265

Target: ZNF265 (Zranb2) (Zinc Finger Protein 265 (Zranb2))

Alternative Name: ZNF265 (Zranb2 Products)

Background: ZNF265 is a protein that has been shown to bind to the spliceosomal components U1-70K and U2AF35 and to direct alternative splicing. Analysis of the structure reveals substantial similarity to known RNA-binding motifs in terms of the distribution of key surface residues responsible for making RNA contacts, despite a complete lack of structural homology. An RNA gel shift assay was used to demonstrate that a single crossed finger domain from ZNF265 is capable of binding to an RNA message. Taken together, these results define a new RNA-binding motif and should provide insight into the functions of the >100 uncharacterized proteins in the sequence data bases that contain this domain.
Alias Symbols: ZIS, ZIS1, ZIS2, ZNF265
Protein Interaction Partner: UBC, C18orf25, TBC1D17, CORO1B, PARVA, SWAP70, OSBP, CDK1, FBXL7, SRPK2, SRPK1, CSNK2A1, KIF20A, SMURF1, UBE2B, UBA52, TYMS, TTC1, SULT1A1, SRSF7, PSMA1, PCMT1, UCHL3, UBE2V2, TTC9C, SRXN1, UNK, UBL7, ZNF644, UBQLN1, UBQLN2, UBXN7, TTLL12, UBE2C, AHCYL1,
Protein Size: 330

Molecular Weight: 37 kDa

Gene ID: 9406

NCBI Accession: NM_203350, NP_976225

UniProt: Q5VV34