ZNF265 antibody (N-Term)

Catalog No. ABIN2780239

Product Details anti-ZNF265 Antibody

Target: ZNF265 (Zranb2) (Zinc Finger Protein 265 (Zranb2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZNF265 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: RCGREKTTEA KMMKAGGTEI GKTLAEKSRG LFSANDWQCK TCSNVNWARR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against ZNF265. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ZNF265

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 330 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ZNF265 antibody (ABIN2780239)

Wang, Du, Li, Zhang: "Identification of the Zinc Finger Protein ZRANB2 as a Novel Maternal Lipopolysaccharide-binding Protein That Protects Embryos of Zebrafish against Gram-negative Bacterial Infections." in: The Journal of biological chemistry, Vol. 291, Issue 8, pp. 4019-34, (2016) (PubMed).

Target Details for ZNF265

Target: ZNF265 (Zranb2) (Zinc Finger Protein 265 (Zranb2))

Alternative Name: ZNF265 (Zranb2 Products)

Synonyms: ZNF265 antibody, ZRANB2 antibody, c4sr antibody, zis antibody, zis1 antibody, zis2 antibody, znf265 antibody, zranb2 antibody, ZIS antibody, ZIS1 antibody, ZIS2 antibody, AI227013 antibody, Zfp265 antibody, Zis antibody, Znf265 antibody, zinc finger RANBP2-type containing 2 antibody, zinc finger RANBP2-type containing 2 L homeolog antibody, zinc finger, RAN-binding domain containing 2 antibody, ZRANB2 antibody, zranb2.L antibody, Zranb2 antibody

Background: ZNF265 is a protein that has been shown to bind to the spliceosomal components U1-70K and U2AF35 and to direct alternative splicing. Analysis of the structure reveals substantial similarity to known RNA-binding motifs in terms of the distribution of key surface residues responsible for making RNA contacts, despite a complete lack of structural homology. An RNA gel shift assay was used to demonstrate that a single crossed finger domain from ZNF265 is capable of binding to an RNA message. Taken together, these results define a new RNA-binding motif and should provide insight into the functions of the >100 uncharacterized proteins in the sequence data bases that contain this domain.
Alias Symbols: ZIS, ZIS1, ZIS2, ZNF265
Protein Interaction Partner: UBC, C18orf25, TBC1D17, CORO1B, PARVA, SWAP70, OSBP, CDK1, FBXL7, SRPK2, SRPK1, CSNK2A1, KIF20A, SMURF1, UBE2B, UBA52, TYMS, TTC1, SULT1A1, SRSF7, PSMA1, PCMT1, UCHL3, UBE2V2, TTC9C, SRXN1, UNK, UBL7, ZNF644, UBQLN1, UBQLN2, UBXN7, TTLL12, UBE2C, AHCYL1,
Protein Size: 330

Molecular Weight: 37 kDa

Gene ID: 9406

NCBI Accession: NM_203350, NP_976225

UniProt: Q5VV34