G3BP1 antibody (N-Term)

Catalog No. ABIN2780221

Product Details anti-G3BP1 Antibody

Target: G3BP1 (GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Horse, Guinea Pig, Cow, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This G3BP1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: EVFGGFVTEP QEESEEEVEE PEERQQTPEV VPDDSGTFYD QAVVSNDMEE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against G3BP. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human G3BP

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 466 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-G3BP1 antibody (ABIN2780221)

Lind, Svedin, Domsgen, Kapell, Laitinen, Moll, Flodström-Tullberg: "Coxsackievirus counters the host innate immune response by blocking type III interferon expression." in: The Journal of general virology, Vol. 97, Issue 6, pp. 1-12, (2016) (PubMed).

Schulte, Liu, Panas, Thaa, Dickson, Götte, Achour, McInerney: "Combined structural, biochemical and cellular evidence demonstrates that both FGDF motifs in alphavirus nsP3 are required for efficient replication." in: Open biology, Vol. 6, Issue 7, (2016) (PubMed).

Target Details for G3BP1

Target: G3BP1 (GTPase Activating Protein (SH3 Domain) Binding Protein 1 (G3BP1))

Alternative Name: G3BP (G3BP1 Products)

Background: G3BP is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Alias Symbols: G3BP, HDH-VIII
Protein Interaction Partner: UBC, USP10, FXR2, MDM2, RNF2, BMI1, RPS27L, EDC4, RPL35, RPL14, EIF2B3, EIF3C, PTBP1, PIN1, PA2G4, YBX1, HNRNPU, FAU, SH3RF2, TARDBP, FBXO25, RIOK2, SND1, VCAM1, env, ITGA4, IL7R, FN1, EPHA8, DTX1, PABPC1, PAXIP1, ESR1, APP, NOLC1, TPM4, LCK, CSK, KCND3,
Protein Size: 466

Molecular Weight: 51 kDa

Gene ID: 10146

NCBI Accession: NM_005754, NP_005745

UniProt: Q13283

Pathways: SARS-CoV-2 Protein Interactome