TSG101 antibody (Middle Region)

Catalog No. ABIN2780037

Product Details anti-TSG101 Antibody

Target: TSG101 (Tumor Susceptibility Gene 101 (TSG101))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Goat, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TSG101 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: RSELLELIQI MIVIFGEEPP VFSRPTVSAS YPPYTATGPP NTSYMPGMPS

Predicted Reactivity: Cow: 93%, Dog: 86%, Goat: 93%, Guinea Pig: 93%, Horse: 79%, Human: 79%, Mouse: 100%, Rabbit: 79%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TSG101. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide corresponding to a region of Mouse

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 391 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TSG101 antibody (ABIN2780037)

Bychkov, Kirichenko, Mikhaylova, Paramonov, Yastremsky, Kirpichnikov, Shulepko, Lyukmanova: "Extracellular Vesicles Derived from Acidified Metastatic Melanoma Cells Stimulate Growth, Migration, and Stemness of Normal Keratinocytes." in: , Vol. 10, Issue 3, (2022) (PubMed).

Bychkov, Kirichenko, Mikhaylova, Paramonov, Kirpichnikov, Shulepko, Lyukmanova: "Extracellular Vesicles Derived from Metastatic Melanoma Cells Transfer α7-nAChR mRNA, Thus Increasing the Surface Expression of the Receptor and Stimulating the Growth of Normal Keratinocytes." in: Acta naturae, Vol. 14, Issue 3, pp. 95-99, (2022) (PubMed).

Keysberg, Hertel, Schelletter, Busche, Sochart, Kalinowski, Hoffrogge, Otte, Noll: "Exploring the molecular content of CHO exosomes during bioprocessing." in: Applied microbiology and biotechnology, Vol. 105, Issue 9, pp. 3673-3689, (2021) (PubMed).

Leblanc, Arellano-Anaya, Bernard, Gallay, Provansal, Lehmann, Schaeffer, Raposo, Vilette: "Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission." in: Methods in molecular biology (Clifton, N.J.), Vol. 1545, pp. 153-176, (2016) (PubMed).

Stefan, Claiborn, Stasiek, Chai, Ohta, Longnecker, Greally, Nicholls: "Genetic mapping of putative Chrna7 and Luzp2 neuronal transcriptional enhancers due to impact of a transgene-insertion and 6.8 Mb deletion in a mouse model of Prader-Willi and Angelman syndromes." in: BMC genomics, Vol. 6, pp. 157, (2005) (PubMed).

Target Details for TSG101

Target: TSG101 (Tumor Susceptibility Gene 101 (TSG101))

Alternative Name: TSG101 (TSG101 Products)

Background: TSG101 has a direct role in the control of growth and differentiation in primary epithelial cells. It is required for normal cell function of embryonic and adult tissues but that this gene is not a tumor suppressor for sporadic forms of breast cancer.
Alias Symbols: CC2, AI255943
Protein Interaction Partner: Nr3c2, Ndfip1, SH3KBP1, Ppp1cc, Gjd3, Gjb3, Gjc1, Gja1, Ubc, Mgrn1, Tsg101, Nfe2, Ikbkg, Gmcl1,
Protein Size: 391

Molecular Weight: 43 kDa

Gene ID: 22088

NCBI Accession: NM_021884, NP_068684

UniProt: Q61187