Mesp2 antibody (C-Term)

Catalog No. ABIN2779870

Product Details anti-Mesp2 Antibody

Target: Mesp2 (Mesoderm Posterior 2 Homolog (Mesp2))

Binding Specificity: C-Term

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Mesp2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: SCQRLQPQPQ PQPQWGCWGH DAEVLSTSED QGSSPALQLP VASPTPSSGL

Predicted Reactivity: Mouse: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against MESP2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of mouse MESP2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 370 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Mesp2 antibody (ABIN2779870)

Leschik, Stefanovic, Brinon, Pucéat: "Cardiac commitment of primate embryonic stem cells." in: Nature protocols, Vol. 3, Issue 9, pp. 1381-7, (2008) (PubMed).

Target Details for Mesp2

Target: Mesp2 (Mesoderm Posterior 2 Homolog (Mesp2))

Alternative Name: MESP2 (Mesp2 Products)

Synonyms: bHLHc6 antibody, SCDO2 antibody, mesoderm posterior 2 antibody, mesoderm posterior bHLH transcription factor 2 antibody, Mesp2 antibody, MESP2 antibody

Background: Somite boundaries formed during embryogenesis, at the interface between Notch1-activated and -repressed domains. Genetic and biochemical studies indicate that this interface is generated by suppression of Notch activity by mesoderm posterior 2 (Mesp2) through induction of the lunatic fringe gene (Lfng). It is proposed that the oscillation of Notch activity is arrested and translated in the wavefront by Mesp2.
Alias Symbols: bHLHc6
Protein Interaction Partner: Lfng,
Protein Size: 370

Molecular Weight: 41 kDa

Gene ID: 17293

NCBI Accession: NM_008589, NP_032615

UniProt: O08574