HIPK2 antibody (Middle Region)

Catalog No. ABIN2779735

Product Details anti-HIPK2 Antibody

Target: HIPK2 (Homeodomain Interacting Protein Kinase 2 (HIPK2))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIPK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: MWSLGCVIAE LFLGWPLYPG ASEYDQIRYI SQTQGLPAEY LLSAGTKTTR

Predicted Reactivity: Cow: 86%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 87%

Characteristics: This is a rabbit polyclonal antibody against HIPK2. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human HIPK2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1198 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HIPK2 antibody (ABIN2779735)

Upadhyay, Bhadauriya, Ganesh: "Heat shock modulates the subcellular localization, stability, and activity of HIPK2." in: Biochemical and biophysical research communications, Vol. 472, Issue 4, pp. 580-4, (2016) (PubMed).

Steinmann, Schulte, Beck, Chachra, Bujnicki, Klempnauer: "v-Myc inhibits C/EBPbeta activity by preventing C/EBPbeta-induced phosphorylation of the co-activator p300." in: Oncogene, Vol. 28, Issue 26, pp. 2446-55, (2009) (PubMed).

Choi, Seo, Kim, Sung, Ahn, Park, Lee, Choi: "Ubiquitination and degradation of homeodomain-interacting protein kinase 2 by WD40 repeat/SOCS box protein WSB-1." in: The Journal of biological chemistry, Vol. 283, Issue 8, pp. 4682-9, (2008) (PubMed).

Target Details for HIPK2

Target: HIPK2 (Homeodomain Interacting Protein Kinase 2 (HIPK2))

Alternative Name: HIPK2 (HIPK2 Products)

Synonyms: HIPK2 antibody, cb1009 antibody, si:dkey-16j24.2 antibody, si:dkey-239k7.1 antibody, wu:fk56d12 antibody, pro0593 antibody, MGC146781 antibody, PRO0593 antibody, 1110014O20Rik antibody, B230339E18Rik antibody, Stank antibody, PKM antibody, homeodomain interacting protein kinase 2 antibody, serine/threonine kinase antibody, homeodomain interacting protein kinase 2 L homeolog antibody, HIPK2 antibody, Smp_000720 antibody, hipk2 antibody, hipk2.L antibody, Hipk2 antibody

Background: HIPK2 is a conserved serine/threonine nuclear kinase that interacts with homeodomain transcription factors.
Alias Symbols: DKFZp686K02111, FLJ23711, PRO0593
Protein Interaction Partner: UBC, TP53, SIAH2, XAF1, AATF, SUMO1, MDM2, AXIN1, SIAH1, MBP, DAXX, MKNK1, MECP2, ZBTB4, HIPK2, DCAF7, DYRK1B, MAP3K1, DYRK1A, FBXO3, CBX4, SUMO2, SUMO3, PML, PAX6, CHMP4B, SENP2, SENP1, UBE2I, HIST2H2BE, CXCL1, SIAH1P1, WSB1, MYBL2, MYBL1, NLK, MAP3K7, M
Protein Size: 1198

Molecular Weight: 120 kDa

Gene ID: 28996

NCBI Accession: NM_022740, NP_073577

UniProt: Q9H2X6

Pathways: Cell Division Cycle