EHMT2 antibody (N-Term)

Catalog No. ABIN2779680

Product Details anti-EHMT2 Antibody

Target: EHMT2 (Ankyrin Repeat-Containing Protein (EHMT2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EHMT2 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Sequence: VQSLAMRLLS MPGAQGAAAA GSEPPPATTS PEGQPKVHRA RKTMSKPGNG

Predicted Reactivity: Cow: 92%, Dog: 86%, Guinea Pig: 93%, Horse: 77%, Human: 100%, Mouse: 79%, Rabbit: 100%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against EHMT2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EHMT2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1210 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EHMT2 antibody (ABIN2779680)

Kondo, Shen, Ahmed, Boumber, Sekido, Haddad, Issa: "Downregulation of histone H3 lysine 9 methyltransferase G9a induces centrosome disruption and chromosome instability in cancer cells." in: PLoS ONE, Vol. 3, Issue 4, pp. e2037, (2008) (PubMed).

Target Details for EHMT2

Target: EHMT2 (Ankyrin Repeat-Containing Protein (EHMT2))

Alternative Name: EHMT2 (EHMT2 Products)

Background: A cluster of genes, BAT1-BAT5, has been localized in the vicinity of the genes for TNF alpha and TNF beta. This gene is found near this cluster, it was mapped near the gene for C2 within a 120-kb region that included a HSP70 gene pair. These genes are all within the human major histocompatibility complex class III region. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but a recent publication shows that there is only a single gene. The protein encoded by this gene is thought to be involved in intracellular protein-protein interaction.A cluster of genes, BAT1-BAT5, has been localized in the vicinity of the genes for TNF alpha and TNF beta. This gene is found near this cluster, it was mapped near the gene for C2 within a 120-kb region that included a HSP70 gene pair. These genes are all within the human major histocompatibility complex class III region. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but a recent publication shows that there is only a single gene. The protein encoded by this gene is thought to be involved in intracellular protein-protein interaction. There are three alternatively spliced transcript variants of this gene but only two are fully described.
Alias Symbols: BAT8, C6orf30, DKFZp686H08213, FLJ35547, G9A, KMT1C, NG36, NG36/G9a, GAT8
Protein Interaction Partner: PLOD3, NAA10, PSMA1, MX2, ITGB5, RD3, ZBTB38, ZNF785, NAPRT, FAM161A, KLF3, CHCHD2, C10orf12, DNMT1, PML, HIST2H3A, HIST1H1A, PRDM1, XAGE2, EED, RNF2, CTNNA3, SOX2, HDAC1, CBX5, CBX3, CBX1, REST, EHMT1, WIZ, MIER1, EHMT2, CDYL, UBC, HDAC2, HIST2H3C, CUX1,
Protein Size: 1210

Molecular Weight: 132 kDa

Gene ID: 10919

NCBI Accession: NM_006709, NP_006700

UniProt: Q96KQ7