REST antibody (Middle Region)

Catalog No. ABIN2779670

Product Details anti-REST Antibody

Target: REST (RE1-Silencing Transcription Factor (REST))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Mouse, Dog, Guinea Pig, Horse, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This REST antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: PMLPPSAVEE REAVSKTALA SPPATMAANE SQEIDEDEGI HSHEGSDLSD

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against REST. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human REST

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1097 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-REST antibody (ABIN2779670)

Mehta, Kim, Vemuganti: "Long Noncoding RNA FosDT Promotes Ischemic Brain Injury by Interacting with REST-Associated Chromatin-Modifying Proteins." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 50, pp. 16443-9, (2015) (PubMed).

Target Details for REST

Target: REST (RE1-Silencing Transcription Factor (REST))

Alternative Name: REST (REST Products)

Background: REST is a transcriptional repressor which represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells, and it is thought that this repressor may act as a master negative regular of neurogenesis.This gene encodes a transcriptional repressor which represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells, and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described, however, their full length nature has not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: NRSF, XBR
Protein Interaction Partner: BTRC, UBC, FBXW11, CSNK1E, NEDD8, EHMT2, EIF4A3, FBXW4, FBXW7, FBXW5, FBXW8, FBXW2, SIN3A, TERF2, GRIA2, SUB1, GTF2B, Tbp, SIN3B, HDAC1, RCOR1, SP1, SMARCE1, SMARCC2, SMARCA4, CDYL, HDAC5, HDAC4, HTT, NCOR1, KDM5C, RNF2, HDAC2, STMN2, GRM2, CTDSP1,
Protein Size: 1097

Molecular Weight: 122 kDa

Gene ID: 5978

NCBI Accession: NM_005612, NP_005603

UniProt: Q13127

Pathways: Negative Regulation of Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Chromatin Binding, Positive Regulation of Endopeptidase Activity