HES1 antibody (N-Term)

Catalog No. ABIN2779597

Product Details anti-HES1 Antibody

Target: HES1 (Hes Family bHLH Transcription Factor 1 (HES1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HES1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Sequence: NTTPDKPKTA SEHRKSSKPI MEKRRRARIN ESLSQLKTLI LDALKKDSSR

Predicted Reactivity: Cow: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against HES1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HES1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 280 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HES1 antibody (ABIN2779597)

Sun, Zhou, Liu, Zhang, Chen, Pan, Ma, Liu, Du, Yang, Wang: "Inhibition of breast cancer cell survival by Xanthohumol via modulation of the Notch signaling pathway in vivo and in vitro." in: Oncology letters, Vol. 15, Issue 1, pp. 908-916, (2018) (PubMed).

Natsumeda, Maitani, Liu, Miyahara, Kaur, Chu, Zhang, Kahlert, Eberhart: "Targeting Notch Signaling and Autophagy Increases Cytotoxicity in Glioblastoma Neurospheres." in: Brain pathology (Zurich, Switzerland), Vol. 26, Issue 6, pp. 713-723, (2015) (PubMed).

Meng, Su, Liu, Wang, Wang: "Rac1 contributes to cerebral ischemia reperfusion-induced injury in mice by regulation of Notch2." in: Neuroscience, Vol. 306, pp. 100-14, (2015) (PubMed).

Ma, Mao, Shen, Zheng, Li, Liu, Ni: "Atractylenolide I-mediated Notch pathway inhibition attenuates gastric cancer stem cell traits." in: Biochemical and biophysical research communications, Vol. 450, Issue 1, pp. 353-9, (2014) (PubMed).

Asnaghi, Lin, Lim, Lim, Tripathy, Wendeborn, Merbs, Handa, Sodhi, Bar, Eberhart: "Hypoxia promotes uveal melanoma invasion through enhanced Notch and MAPK activation." in: PLoS ONE, Vol. 9, Issue 8, pp. e105372, (2014) (PubMed).

Guiu, Shimizu, DAltri, Fraser, Hatakeyama, Bresnick, Kageyama, Dzierzak, Yamamoto, Espinosa, Bigas: "Hes repressors are essential regulators of hematopoietic stem cell development downstream of Notch signaling." in: The Journal of experimental medicine, Vol. 210, Issue 1, pp. 71-84, (2013) (PubMed).

Chu, Liu, Wang: "Overexpression of hCLP46 enhances Notch activation and regulates cell proliferation in a cell type-dependent manner." in: Cell proliferation, Vol. 46, Issue 3, pp. 254-62, (2013) (PubMed).

Asnaghi, Ebrahimi, Schreck, Bar, Coonfield, Bell, Handa, Merbs, Harbour, Eberhart: "Notch signaling promotes growth and invasion in uveal melanoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 18, Issue 3, pp. 654-65, (2012) (PubMed).

Ma, Du, Chu, Wang, Liu, Song, Wang: "hCLP46 regulates U937 cell proliferation via Notch signaling pathway." in: Biochemical and biophysical research communications, Vol. 408, Issue 1, pp. 84-8, (2011) (PubMed).

Bennani-Baiti, Aryee, Ban, Machado, Kauer, Mühlbacher, Amann, Llombart-Bosch, Kovar: "Notch signalling is off and is uncoupled from HES1 expression in Ewing's sarcoma." in: The Journal of pathology, Vol. 225, Issue 3, pp. 353-63, (2011) (PubMed).

Schreck, Taylor, Marchionni, Gopalakrishnan, Bar, Gaiano, Eberhart: "The Notch target Hes1 directly modulates Gli1 expression and Hedgehog signaling: a potential mechanism of therapeutic resistance." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 16, Issue 24, pp. 6060-70, (2010) (PubMed).

Tilley, Harvey, Heguy, Hackett, Wang, OConnor, Crystal: "Down-regulation of the notch pathway in human airway epithelium in association with smoking and chronic obstructive pulmonary disease." in: American journal of respiratory and critical care medicine, Vol. 179, Issue 6, pp. 457-66, (2009) (PubMed).

Target Details for HES1

Target: HES1 (Hes Family bHLH Transcription Factor 1 (HES1))

Alternative Name: HES1 (HES1 Products)

Synonyms: HES-1 antibody, HHL antibody, HRY antibody, bHLHb39 antibody, Hry antibody, HAIRY1 antibody, c-hairy1A antibody, Xhairy1 antibody, hairy1 antibody, hes1 antibody, Xhairy-1 antibody, Xlh1 antibody, hes family bHLH transcription factor 1 antibody, hairy and enhancer of split 1 (Drosophila) antibody, hes family bHLH transcription factor 4 antibody, hes family bHLH transcription factor 1 S homeolog antibody, hes family bHLH transcription factor 1 L homeolog antibody, HES1 antibody, Hes1 antibody, HES4 antibody, hes1.S antibody, hes1.L antibody

Background: HES1 belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
Alias Symbols: HHL, HRY, HES-1, bHLHb39
Protein Interaction Partner: STAT3, JAK2, APH1A, LTBR, ASGR2, TLE1, ELAVL1, PARP1, UBC, NUDT3, HDAC6, YWHAB, PTK2, NR4A1, HMGCL, GAPDH, FHL1, CSNK1E, APCS, FANCE, FANCC, FANCA, FANCL, SPTAN1, HSP90AA1, HSPA4, FANCG, FANCF, HDAC1, RUNX2, HES6, ID4, ID1, SIRT1, UBQLN1, HEY2, ID2, ID3,
Protein Size: 280

Molecular Weight: 30 kDa

Gene ID: 3280

NCBI Accession: NM_005524, NP_005515

UniProt: Q14469

Pathways: DNA Damage Repair