GLI1 antibody (C-Term)

Catalog No. ABIN2779593

Product Details anti-GLI1 Antibody

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Guinea Pig, Horse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: TNPSCGHPEV GRLGGGPALY PPPEGQVCNP LDSLDLDNTQ LDFVAILDEP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against GLI1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human GLI1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1106 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GLI1 antibody (ABIN2779593)

Bosco-Clément, Zhang, Chen, Zhou, Li, Mikami, Hirata, Yagui-Beltran, Lui, Do, Cheng, Tseng, Choi, Fang, Kim, Yue, Wang, Zheng, Fujii, Mann, Jablons, He: "Targeting Gli transcription activation by small molecule suppresses tumor growth." in: Oncogene, Vol. 33, Issue 16, pp. 2087-97, (2014) (PubMed).

Pandolfi, Montagnani, Penachioni, Vinci, Olivito, Borgognoni, Stecca: "WIP1 phosphatase modulates the Hedgehog signaling by enhancing GLI1 function." in: Oncogene, Vol. 32, Issue 40, pp. 4737-47, (2013) (PubMed).

Iwata, Suzuki, Pelikan, Ho, Sanchez-Lara, Chai: "Modulation of lipid metabolic defects rescues cleft palate in Tgfbr2 mutant mice." in: Human molecular genetics, Vol. 23, Issue 1, pp. 182-93, (2013) (PubMed).

Ortega, Cases, Merchán, Kozyraki, Clemente, de Castro: "Megalin mediates the influence of sonic hedgehog on oligodendrocyte precursor cell migration and proliferation during development." in: Glia, Vol. 60, Issue 6, pp. 851-66, (2012) (PubMed).

Ferruzzi, Mennillo, De Rosa, Giordano, Rossi, Benedetti, Magrini, Pericot Mohr, Miragliotta, Magnoni, Mori, Thomas, Tunici, Bakker: "In vitro and in vivo characterization of a novel Hedgehog signaling antagonist in human glioblastoma cell lines." in: International journal of cancer. Journal international du cancer, Vol. 131, Issue 2, pp. E33-44, (2012) (PubMed).

Maitah, Ali, Ahmad, Gadgeel, Sarkar: "Up-regulation of sonic hedgehog contributes to TGF-?1-induced epithelial to mesenchymal transition in NSCLC cells." in: PLoS ONE, Vol. 6, Issue 1, pp. e16068, (2011) (PubMed).

Schiapparelli, Shahi, Enguita-Germán, Johnsen, Kogner, Lázcoz, Castresana: "Inhibition of the sonic hedgehog pathway by cyplopamine reduces the CD133+/CD15+ cell compartment and the in vitro tumorigenic capability of neuroblastoma cells." in: Cancer letters, Vol. 310, Issue 2, pp. 222-31, (2011) (PubMed).

Yoshimura, Kawate, Takeda: "Signaling through the primary cilium affects glial cell survival under a stressed environment." in: Glia, Vol. 59, Issue 2, pp. 333-44, (2010) (PubMed).

Target Details for GLI1

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Alternative Name: GLI1 (GLI1 Products)

Synonyms: GLI antibody, Gli antibody, Gli-1 antibody, gli1.1 antibody, AV235269 antibody, Zfp-5 antibody, Zfp5 antibody, GLI family zinc finger 1 antibody, GLI family zinc finger 1 S homeolog antibody, GLI-Kruppel family member GLI1 antibody, GLI1 antibody, Gli1 antibody, gli1.S antibody, gli1 antibody

Background: GLI1 is a protein which is a member of the Kruppel family of zinc finger proteins. The function of this gene has not been determined, however, it may play a role in normal development gene transcription. Mouse mutation studies indicate possible involvement in human foregut malformation.
Alias Symbols: GLI
Protein Interaction Partner: TRIM42, GCC1, PRKAA2, VHL, FEM1B, CDK4, UBC, MYC, ITCH, NUMB, SUFU, RAI1, XBP1, HDAC2, HDAC1, STK36, DYRK1A, ZIC2, ZIC1, Sin3a, Shh, SAP18, Su(fu), Pias1,
Protein Size: 1106

Molecular Weight: 118 kDa

Gene ID: 2735

NCBI Accession: NM_005269, NP_005260

UniProt: P08151

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis