ASCL1 antibody (N-Term)

Catalog No. ABIN2779585

Product Details anti-ASCL1 Antibody

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASCL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: QSAQQQQQQQ QQQQQAPQLR PAADGQPSGG GHKSAPKQVK RQRSSSPELM

Predicted Reactivity: Cow: 100%, Dog: 92%, Guinea Pig: 100%, Human: 100%, Mouse: 92%, Rabbit: 92%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against ASCL1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ASCL1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 236 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ASCL1 antibody (ABIN2779585)

Suozzi, Malatesta, Zancanaro: "Subcellular distribution of key enzymes of lipid metabolism during the euthermia-hibernation-arousal cycle." in: Journal of anatomy, Vol. 214, Issue 6, pp. 956-62, (2009) (PubMed).

Vias, Massie, East, Scott, Warren, Zhou, Nikitin, Neal, Mills: "Pro-neural transcription factors as cancer markers." in: BMC medical genomics, Vol. 1, pp. 17, (2008) (PubMed).

Target Details for ASCL1

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Alternative Name: ASCL1 (ASCL1 Products)

Synonyms: ASH1 antibody, HASH1 antibody, MASH1 antibody, bHLHa46 antibody, AI225900 antibody, Mash1 antibody, ASCL1 antibody, Zash-1a antibody, ash antibody, asha antibody, id:ibd5085 antibody, im:7138587 antibody, si:dkey-264k15.2 antibody, zash1a antibody, zasha antibody, Xash1 antibody, ascl1-A antibody, Zash-1b antibody, ashb antibody, zashb antibody, achaete-scute family bHLH transcription factor 1 antibody, achaete-scute family bHLH transcription factor 1a antibody, achaete-scute family bHLH transcription factor 1 L homeolog antibody, achaete-scute complex homolog 1 antibody, achaete-scute family bHLH transcription factor 1b antibody, ASCL1 antibody, Ascl1 antibody, ascl1a antibody, ascl1.L antibody, LOC100220505 antibody, ascl1b antibody

Background: ASCL1 encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. It is highly expressed in medullary thyroid cancer and small cell lung cancer and may be a useful marker for these cancers.
Alias Symbols: ASH1, HASH1, MASH1, bHLHa46
Protein Interaction Partner: MEF2D, MEF2C, MEF2A, NEUROG2, UBQLN1, EP300, TCF4, BMP2, ASCL1,
Protein Size: 236

Molecular Weight: 25 kDa

Gene ID: 429

NCBI Accession: NM_004316, NP_004307

UniProt: P50553

Pathways: Dopaminergic Neurogenesis