Nkx2-2 antibody (N-Term)

Catalog No. ABIN2779575

Product Details anti-Nkx2-2 Antibody

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nkx2-2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Sequence: MSLTNTKTGF SVKDILDLPD TNDEEGSVAE GPEEENEGPE PAKRAGPLGQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 92%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against NKX2-2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NKX2-2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 273 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Nkx2-2 antibody (ABIN2779575)

Owen, Kowalewski, Lessnick: "EWS/FLI mediates transcriptional repression via NKX2.2 during oncogenic transformation in Ewing's sarcoma." in: PLoS ONE, Vol. 3, Issue 4, pp. e1965, (2008) (PubMed).

Target Details for Nkx2-2

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Alternative Name: NKX2-2 (Nkx2-2 Products)

Synonyms: XeNK-2 antibody, Xnkx2.2 antibody, nkx2-2-A antibody, nkx2.2 antibody, NKX-2.2 antibody, NKX2.2 antibody, NKX2B antibody, Nkx-2.2 antibody, Nkx2.2 antibody, tinman antibody, id:ibd5090 antibody, nk2.2 antibody, nkx2-2 antibody, wu:fc83b01 antibody, zgc:136536 antibody, NK2 homeobox 2 S homeolog antibody, NK2 homeobox 2 antibody, NK2 homeobox 2a antibody, nkx2-2.S antibody, NKX2-2 antibody, Nkx2-2 antibody, nkx2.2a antibody

Background: Nkx2-2 contains 1 homeobox DNA-binding domain which is essential for interaction with OLIG2. Nkx2-2 may be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. The protein encoded by this gene contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor.
Alias Symbols: NKX2.2, NKX2B
Protein Interaction Partner: Dlg4, SIN3A, HDAC1, OLIG2,
Protein Size: 273

Molecular Weight: 30 kDa

Gene ID: 4821

NCBI Accession: NM_002509, NP_002500

UniProt: O95096

Pathways: Dopaminergic Neurogenesis