EGR1 antibody (N-Term)

Catalog No. ABIN2779517

Product Details anti-EGR1 Antibody

Target: EGR1 (Early Growth Response 1 (EGR1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Horse, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: DNYPKLEEMM LLSNGAPQFL GAAGAPEGSG SNSSSSSSGG GGGGGGGSNS

Predicted Reactivity: Cow: 87%, Horse: 93%, Human: 100%, Mouse: 86%, Pig: 100%, Rabbit: 86%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against EGR1. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EGR1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 543 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EGR1 antibody (ABIN2779517)

Balakrishnan, Stykel, Touahri, Stratton, Biernaskie, Schuurmans: "Temporal Analysis of Gene Expression in the Murine Schwann Cell Lineage and the Acutely Injured Postnatal Nerve." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153256, (2016) (PubMed).

Ohata, Yamazaki, Kawai, Tsugawa, Tachikawa, Koinuma, Miyagawa, Kimoto, Nakayama, Namba, Yamamoto, Okano, Ozono, Michigami: "Elevated fibroblast growth factor 23 exerts its effects on placenta and regulates vitamin D metabolism in pregnancy of Hyp mice." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 29, Issue 7, pp. 1627-38, (2014) (PubMed).

Lane, Yu, Lackey, Chen, Marzluff, Cook: "Cell cycle-regulated protein abundance changes in synchronously proliferating HeLa cells include regulation of pre-mRNA splicing proteins." in: PLoS ONE, Vol. 8, Issue 3, pp. e58456, (2013) (PubMed).

Revest, Kaouane, Mondin, Le Roux, Rougé-Pont, Vallée, Barik, Tronche, Desmedt, Piazza: "The enhancement of stress-related memory by glucocorticoids depends on synapsin-Ia/Ib." in: Molecular psychiatry, Vol. 15, Issue 12, pp. 1125, 1140-51, (2010) (PubMed).

Jaluria, Konstantopoulos, Betenbaugh, Shiloach: "Egr1 and Gas6 facilitate the adaptation of HEK-293 cells to serum-free media by conferring enhanced viability and higher growth rates." in: Biotechnology and bioengineering, Vol. 99, Issue 6, pp. 1443-52, (2008) (PubMed).

Wenzel, Daskalow, Herse, Seitz, Zacharias, Schenk, Schulz, Hubner, Micheel, Schlag, Osterziel, Ozcelik, Scherneck, Jandrig: "Expression of the protein phosphatase 1 inhibitor KEPI is downregulated in breast cancer cell lines and tissues and involved in the regulation of the tumor suppressor EGR1 via the MEK-ERK pathway." in: Biological chemistry, Vol. 388, Issue 5, pp. 489-95, (2007) (PubMed).

Barbolina, Adley, Ariztia, Liu, Stack: "Microenvironmental regulation of membrane type 1 matrix metalloproteinase activity in ovarian carcinoma cells via collagen-induced EGR1 expression." in: The Journal of biological chemistry, Vol. 282, Issue 7, pp. 4924-31, (2007) (PubMed).

Target Details for EGR1

Target: EGR1 (Early Growth Response 1 (EGR1))

Alternative Name: EGR1 (EGR1 Products)

Synonyms: AT225 antibody, G0S30 antibody, KROX-24 antibody, NGFI-A antibody, TIS8 antibody, ZIF-268 antibody, ZNF225 antibody, A530045N19Rik antibody, ETR103 antibody, Egr-1 antibody, Krox-1 antibody, Krox-24 antibody, Krox24 antibody, NGF1-A antibody, NGFIA antibody, Zenk antibody, Zfp-6 antibody, Zif268 antibody, egr antibody, Ngf1 antibody, Ngfi antibody, zif-268 antibody, EGR1 antibody, EGR-1-A antibody, Xegr-1 antibody, at225 antibody, egr-1 antibody, egr1 antibody, g0s30 antibody, krox-24 antibody, ngfi-a antibody, tis8 antibody, znf225 antibody, krox24 antibody, wu:fj64b05 antibody, wu:fq25f01 antibody, EGR-1 antibody, zenk antibody, EGR-1-B antibody, egr1-a antibody, egr1-b antibody, early growth response 1 antibody, early growth response protein 1 (egr-1) antibody, early growth response 1 L homeolog antibody, early growth response 1 S homeolog antibody, EGR1 antibody, CNK00890 antibody, Egr1 antibody, egr1.L antibody, egr1 antibody, egr1.S antibody

Background: Early Growth Response Protein 1 (EGR1, Krox-24 protein, nerve growth factor-induced protein A, Transcription factor ETR103, Zinc finger protein 225) belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: AT225, G0S30, KROX-24, NGFI-A, TIS8, ZIF-268, ZNF225
Protein Interaction Partner: CEBPB, WT1, SUMO1, TP53, MDM2, EP300, CDKN2A, SRA1, IL1B, PTEN, SP1, SNAI1, TBX2, PFDN5, SREBF2, ERBB3, NAB2, PITX1, NAB1, NFATC2, CREBBP, PSMA3, JUN, RELA, CSNK2A1, NFATC1, GADD45B, GADD45A,
Protein Size: 543

Molecular Weight: 57 kDa

Gene ID: 1958

NCBI Accession: NM_001964, NP_001955

UniProt: P18146

Pathways: Regulation of Carbohydrate Metabolic Process, Regulation of long-term Neuronal Synaptic Plasticity