E2F6 antibody (Middle Region)

Catalog No. ABIN2779510

Product Details anti-E2F6 Antibody

Target: E2F6 (E2F Transcription Factor 6 (E2F6))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: HEQIVIAVKA PAETRLDVPA PREDSITVHI RSTNGPIDVY LCEVEQGQTS

Predicted Reactivity: Cow: 92%, Dog: 100%, Guinea Pig: 85%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against E2F6. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human E2F6

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 129 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-E2F6 antibody (ABIN2779510)

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, (2006) (PubMed).

Target Details for E2F6

Target: E2F6 (E2F Transcription Factor 6 (E2F6))

Alternative Name: E2F6 (E2F6 Products)

Synonyms: E2F-6 antibody, AI462434 antibody, E2F6b antibody, EMA antibody, E2F transcription factor 6 antibody, E2F6 antibody, E2f6 antibody

Background: E2F6 is a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants encoding several different isoforms have been found for this gene.
Protein Interaction Partner: TFDP2, TFDP1, KRTAP10-7, LYN, ARAF, APP, PCGF6, RYBP, YAF2, RNF2, SMARCAD1, UBC, L3MBTL2, EZH2, Epc1, EED, KMT2D, KMT2A, RING1, E2F1, BMI1, ZMYND8, ZMYND11, KDM5C, EZH1, CBX3, E2F6,
Protein Size: 129

Molecular Weight: 14 kDa

Gene ID: 1876

NCBI Accession: NM_212540, NP_997705

UniProt: Q6Q9Z5