AKT2 antibody (Middle Region)

Catalog No. ABIN2779323

Product Details anti-AKT2 Antibody

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: TSEVDTRYFD DEFTAQSITI TPPDRYDSLG LLELDQRTHF PQFSYSASIR

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against AKT2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human AKT2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 481 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-AKT2 antibody (ABIN2779323)

Tan, Timakhov, Rao, Altomare, Xu, Liu, Gao, Jhanwar, Di Cristofano, Wiest, Knepper, Testa: "A novel recurrent chromosomal inversion implicates the homeobox gene Dlx5 in T-cell lymphomas from Lck-Akt2 transgenic mice." in: Cancer research, Vol. 68, Issue 5, pp. 1296-302, (2008) (PubMed).

Target Details for AKT2

Target: AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))

Alternative Name: AKT2 (AKT2 Products)

Synonyms: AKT2 antibody, pkbb antibody, pkbbeta antibody, pkbss antibody, prkbb antibody, rac-beta antibody, v-akt2 antibody, 2410016A19Rik antibody, AW554154 antibody, PKB antibody, PKBbeta antibody, HIHGHH antibody, PKBB antibody, PKBBETA antibody, PRKBB antibody, RAC-BETA antibody, cb945 antibody, akt2-a antibody, AKT serine/threonine kinase 2 antibody, v-akt murine thymoma viral oncogene homolog 2 antibody, thymoma viral proto-oncogene 2 antibody, v-akt murine thymoma viral oncogene homolog 2 L homeolog antibody, AKT2 antibody, akt2 antibody, Akt2 antibody, akt2.L antibody

Background: AKT2 belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene encoding AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: PKBBETA, PRKBB, RAC-BETA, PKBB, HIHGHH
Protein Interaction Partner: CLIP3, CHUK, PLEKHO1, PRKCZ, UBC, EGFR, POLR1B, SNX27, PNPLA3, STEAP4, ABCG8, POFUT1, TMED2, NAMPT, SORBS2, SPRR2A, CCL14, PRKDC, MAP3K5, HSP90AA1, APOB, APOA1, APPL1, APP, FSHR, VIM, SKI, GRK5, SH3RF1, ESR1, TTC3, SETDB1, XIAP, AKT1S1, TRIB3, PDPK1, GSK3
Protein Size: 481

Molecular Weight: 56 kDa

Gene ID: 208

NCBI Accession: NM_001626, NP_001617

UniProt: P31751

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling