RAD17 antibody (C-Term)
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- Target See all RAD17 Antibodies
- RAD17
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse, Rat, Dog, Cow, Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RAD17 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- PTQATVPETW SLPLSQNSAS ELPASQPQPF SAQGDMEENI IIEDYESDGT
- Predicted Reactivity
- Cow: 83%, Dog: 75%, Human: 100%, Mouse: 75%, Pig: 83%, Rat: 75%
- Characteristics
- This is a rabbit polyclonal antibody against RAD17. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Protein A purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the C terminal region of human RAD17
- Top Product
- Discover our top product RAD17 Primary Antibody
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 584 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Interaction between human MCM7 and Rad17 proteins is required for replication checkpoint signaling." in: The EMBO journal, Vol. 23, Issue 23, pp. 4660-9, (2004) (PubMed).
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Interaction between human MCM7 and Rad17 proteins is required for replication checkpoint signaling." in: The EMBO journal, Vol. 23, Issue 23, pp. 4660-9, (2004) (PubMed).
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- Target
- RAD17
- Alternative Name
- RAD17 (RAD17 Products)
- Synonyms
- zgc:91969 antibody, RAD17 antibody, K2A18.21 antibody, K2A18_21 antibody, RADIATION SENSITIVE 17 antibody, CCYC antibody, HRAD17 antibody, R24L antibody, RAD17SP antibody, RAD24 antibody, 9430035O09Rik antibody, MmRad24 antibody, RAD17 checkpoint clamp loader component antibody, Rad17p antibody, RADIATION SENSITIVE 17 antibody, RAD17 antibody, rad17 antibody, ATRAD17 antibody, Rad17 antibody
- Background
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RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation.The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported.
Alias Symbols: CCYC, HRAD17, R24L, RAD17Sp, Rad24, RAD24, RAD17SP
Protein Interaction Partner: USP20, UBC, PRMT6, CBX7, CBX6, RAD9A, CDH1, ATR, RFC1, H2AFX, FOXO3, ATM, CLSPN, ALK, RAD9B, POLE, HUS1, RAD1, NHP2L1, MCM7, RFC4, RFC5, RFC3, RFC2, POLE4, POLE3, POLE2, PRKDC,
Protein Size: 584 - Molecular Weight
- 66 kDa
- Gene ID
- 5884
- NCBI Accession
- NM_133341, NP_579919
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