RNP antibody (Middle Region)

Catalog No. ABIN2779114

Product Details anti-RNP Antibody

Target: RNP (RNPC3) (RNA-Binding Region (RNP1, RRM) Containing 3 (RNPC3))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNP antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LHAPLPPTSP QPPEEPPLPD EDEELSSEES EYESTDDEDR QRMNKLMELA

Predicted Reactivity: Cow: 92%, Dog: 92%, Horse: 92%, Human: 100%, Mouse: 85%, Pig: 92%, Rabbit: 92%, Rat: 85%, Yeast: 77%

Characteristics: This is a rabbit polyclonal antibody against RNPC3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RNPC3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 517 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RNP

Target: RNP (RNPC3) (RNA-Binding Region (RNP1, RRM) Containing 3 (RNPC3))

Alternative Name: RNPC3 (RNPC3 Products)

Synonyms: rnp antibody, rbm40 antibody, zgc:136847 antibody, RBM40 antibody, RNP antibody, SNRNP65 antibody, 2810441O16Rik antibody, AI447568 antibody, C030014B17Rik antibody, RNA binding region (RNP1, RRM) containing 3 antibody, RNA-binding region (RNP1, RRM) containing 3 antibody, RNPC3 antibody, rnpc3 antibody, Rnpc3 antibody

Background: RNPC3 is an RNA-binding protein involved in pre-mRNA splicing. RNPC3 participates in pre-mRNA U12-dependent splicing. RNPC3 binds to the 3'-stem-loop of m7G-capped U12 snRNA.Two types of spliceosomes catalyze splicing of pre-mRNAs. The major U2-type spliceosome is found in all eukaryotes and removes U2-type introns, which represent more than 99 % of pre-mRNA introns. The minor U12-type spliceosome is found in some eukaryotes and removes U12-type introns, which are rare and have distinct splice consensus signals. The U12-type spliceosome consists of several small nuclear RNAs and associated proteins. This gene encodes a 65K protein that is a component of the U12-type spliceosome. This protein contains two RNA recognition motifs (RRMs), suggesting that it may contact one of the small nuclear RNAs of the minor spliceosome.
Alias Symbols: FLJ20008, FLJ25070, KIAA183, RBM40, RNP
Protein Interaction Partner: SMAD2, CPSF1, CDK19, RPS3A, APP, PRNP, RNU11, RNU12-2P,
Protein Size: 517

Molecular Weight: 58 kDa

Gene ID: 55599

NCBI Accession: NM_017619, NP_060089

UniProt: Q96LT9