Pre-mRNA Branch Site Protein p14 (SF3B14) (Middle Region) antibody

Catalog No. ABIN2779093

Product Details

Target: Pre-mRNA Branch Site Protein p14 (SF3B14)

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Horse, Dog, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Sequence: HLSGFNVCNR YLVVLYYNAN RAFQKMDTKK KEEQLKLLKE KYGINTDPPK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SF3B14. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SF3B14

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 125 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details

Target: Pre-mRNA Branch Site Protein p14 (SF3B14)

Alternative Name: SF3B14 (SF3B14 Products)

Synonyms: 6030419K15Rik antibody, AV001342 antibody, Sf3b14 antibody, zgc:86708 antibody, HSPC175 antibody, Ht006 antibody, P14 antibody, SAP14 antibody, SF3B14a antibody, splicing factor 3B, subunit 6 antibody, splicing factor 3b, subunit 6 antibody, splicing factor 3b subunit 6 S homeolog antibody, splicing factor 3b subunit 6 antibody, Sf3b6 antibody, sf3b6 antibody, sf3b6.S antibody, SF3B6 antibody

Background: SF3B14 is a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. SF3B14 also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site.This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site.
Alias Symbols: CGI-110, HSPC175, Ht006, P14, SAP14, SF3B14a
Protein Interaction Partner: UBC, WWOX, SUZ12, EED, PAN2, ITGA4, FN1, SF3B1, VCAM1, SF3B5, THOC6, WDR18, TRA2A, PRPF19, RBMX, SF3B3, PUF60, DHX15, RCC1, APP, U2AF2, ZNF326, ZC3H18, FTSJ3, PHF5A, TRIM55, SF3B2, UTP14A, USP39, PRPF8, BCAS2, SF3B4, THRAP3, EFTUD2, SRSF11, SART1, VTN, U2
Protein Size: 125

Molecular Weight: 14 kDa

Gene ID: 51639

NCBI Accession: NM_016047, NP_057131

UniProt: Q7RTV0