AUH antibody (C-Term)

Catalog No. ABIN2778782

Product Details anti-AUH Antibody

Target: AUH (AU RNA Binding Protein/enoyl-CoA Hydratase (AUH))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Dog, Rabbit, Cow, Horse, Saccharomyces cerevisiae, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AUH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: IGMSLAKELI FSARVLDGKE AKAVGLISHV LEQNQEGDAA YRKALDLARE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 75%

Characteristics: This is a rabbit polyclonal antibody against AUH. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human AUH

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 339 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for AUH

Target: AUH (AU RNA Binding Protein/enoyl-CoA Hydratase (AUH))

Alternative Name: AUH (AUH Products)

Synonyms: C77140 antibody, W91705 antibody, wu:fb81b10 antibody, zgc:101057 antibody, AU RNA binding methylglutaconyl-CoA hydratase antibody, AU RNA binding protein/enoyl-coenzyme A hydratase antibody, AU RNA binding protein/enoyl-CoA hydratase antibody, AU RNA binding protein/enoyl-CoA hydratase S homeolog antibody, AUH antibody, Auh antibody, auh antibody, auh.S antibody

Background: AU-specific RNA-binding enoyl-CoA hydratase (AUH) protein binds to the AU-rich element (ARE), a common element found in the 3' UTR of rapidly decaying mRNA such as c-fos, c-myc and granulocyte/ macrophage colony stimulating factor. ARE elements are involved in directing RNA to rapid degradation and deadenylation. AUH is also homologous to enol-CoA hydratase, an enzyme involved in fatty acid degradation, and has been shown to have intrinsic hydratase enzymatic activity. AUH is thus a bifunctional chimera between RNA binding and metabolic enzyme activity. A possible subcellular localization in the mitochondria has been demonstrated for the mouse homolog of this protein which shares 92 % identity with the human protein. It has been suggested that AUH may have a novel role as a mitochondrial located AU-binding protein. Human AUH is expressed as a single mRNA species of 1.8 kb, and translated as a 40- kDa precursor protein which is subsequently processed to a 32- kDa mature form.AU-specific RNA-binding enoyl-CoA hydratase (AUH) protein binds to the AU-rich element (ARE), a common element found in the 3' UTR of rapidly decaying mRNA such as c-fos, c-myc and granulocyte/ macrophage colony stimulating factor. ARE elements are involved in directing RNA to rapid degradation and deadenylation. AUH is also homologous to enol-CoA hydratase, an enzyme involved in fatty acid degradation, and has been shown to have intrinsic hydratase enzymatic activity. AUH is thus a bifunctional chimera between RNA binding and metabolic enzyme activity. A possible subcellular localization in the mitochondria has been demonstrated for the mouse homolog of this protein which shares 92 % identity with the human protein. It has been suggested that AUH may have a novel role as a mitochondrial located AU-binding protein. Human AUH is expressed as a single mRNA species of 1.8 kb, and translated as a 40- kDa precursor protein which is subsequently processed to a 32- kDa mature form.
Protein Interaction Partner: UBC, AUH,
Protein Size: 339

Molecular Weight: 37 kDa

Gene ID: 549

NCBI Accession: NM_001698, NP_001689

UniProt: Q13825