RPS14 antibody (Middle Region)

Catalog No. ABIN2778744

Product Details anti-RPS14 Antibody

Target: RPS14 (Ribosomal Protein S14 (RPS14))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, Rabbit, Guinea Pig, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS14 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GNRTKTPGPG AQSALRALAR SGMKIGRIED VTPIPSDSTR RKGGRRGRRL

Predicted Reactivity: Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against RPS14. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RPS14

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 151 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RPS14

Target: RPS14 (Ribosomal Protein S14 (RPS14))

Alternative Name: RPS14 (RPS14 Products)

Background: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS14 is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor.Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Alias Symbols: S14, EMTB
Protein Interaction Partner: FUS, TAF9, HUWE1, CEP76, TUBG1, CEP250, TUBGCP3, CEP57, TP53, AURKA, UBC, MDM2, ZBTB1, EED, WIBG, TSR1, SERBP1, LARP1, RPS29, RPS28, RPS27, RPS26, RPS25, RPS24, RPS23, RPS20, RPS19, RPS18, RPS16, RPS15A, RPS13, RPS12, RPS10, RPS9, RPS8, RPS7, RPS6, RPS5,
Protein Size: 151

Molecular Weight: 17 kDa

Gene ID: 6208

NCBI Accession: NM_001025071, NP_001020242

UniProt: P62263

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly