SF3B1 antibody (N-Term)

Catalog No. ABIN2778700

Product Details anti-SF3B1 Antibody

Target: SF3B1 (Splicing Factor 3b, Subunit 1, 155kDa (SF3B1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Goat, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SF3B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: MAKIAKTHED IEAQIREIQG KKAALDEAQG VGLDSTGYYD QEIYGGSDSR

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against SF3B1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SF3B1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1304 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SF3B1

Target: SF3B1 (Splicing Factor 3b, Subunit 1, 155kDa (SF3B1))

Alternative Name: SF3B1 (SF3B1 Products)

Background: SF3B1 is subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures.This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.
Alias Symbols: PRP10, SAP155, SF3b155, MDS, Hsh155, PRPF10
Protein Interaction Partner: SART3, SUMO2, SUMO3, MDM2, STAU1, UBC, LGR4, WWOX, RPA3, RPA2, RPA1, ERG, EED, RNF2, EZH2, SUZ12, APBB1, PRPF40A, WBP4, TCERG1, FBXO6, RBM17, SNIP1, SF3B6, WHSC1, RPS2, RPL23A, RPL18A, RPL10, RPL3, PLRG1, SMAD9, SMAD5, SMAD1, IL7R, FN1, ESR1, CSNK2A1, EIF
Protein Size: 1304

Molecular Weight: 143 kDa

Gene ID: 23451

NCBI Accession: NM_012433, NP_036565

UniProt: O75533

Pathways: Chromatin Binding