RPL8 antibody (C-Term)

Catalog No. ABIN2778675

Product Details anti-RPL8 Antibody

Target: RPL8 (Ribosomal Protein L8 (RPL8))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Rabbit, Guinea Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPL8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: KKVISSANRA VVGVVAGGGR IDKPILKAGR AYHKYKAKRN CWPRVRGVAM

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against RPL8. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human RPL8

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 257 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-RPL8 antibody (ABIN2778675)

Wong, Traynor, Basse, Kay, Warren: "Defective ribosome assembly in Shwachman-Diamond syndrome." in: Blood, Vol. 118, Issue 16, pp. 4305-12, (2011) (PubMed).

Target Details for RPL8

Target: RPL8 (Ribosomal Protein L8 (RPL8))

Alternative Name: RPL8 (RPL8 Products)

Synonyms: L8 antibody, zgc:73105 antibody, zgc:77641 antibody, CG1263 antibody, Dmel\\CG1263 antibody, M(3)62F antibody, M(3)LS2 antibody, Rp L8 antibody, anon-EST:Posey5 antibody, rpL8 antibody, RPL8 antibody, ribosomal protein L8 antibody, ribosomal protein L8 S homeolog antibody, Ribosomal protein L8 antibody, microRNA 6850 antibody, RPL8 antibody, Rpl8 antibody, rpl8.S antibody, rpl8 antibody, RpL8 antibody, MIR6850 antibody

Background: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL8 is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm.Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Alias Symbols: L8
Protein Interaction Partner: TP53, HUWE1, SUMO2, TUBGCP3, CEP250, STAU1, UBC, MDM2, ZBTB1, EED, RPS25, RPL28, PARK2, FBXO6, UPF2, TARDBP, ICAM1, IGSF8, PAN2, ITGA4, IL7R, FN1, DPH3, UBL4A, VCAM1, EIF4A3, PA2G4, MAGOH, ESR1, RPLP0P6, RPL10L, TUBA1C, ATF7IP, RPL36, TXN2, PPT2, RPL23, R
Protein Size: 257

Molecular Weight: 28 kDa

Gene ID: 6132

NCBI Accession: NM_000973, NP_000964

UniProt: P62917

Pathways: Regulation of Cell Size