PRMT2 antibody (N-Term)

Catalog No. ABIN2778662

Product Details anti-PRMT2 Antibody

Target: PRMT2 (Protein Arginine Methyltransferase 2 (PRMT2))

Binding Specificity: N-Term

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRMT2 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Sequence: ERAGCCGYIP ANHVGKHVDE YDPEDTWQDE EYFGSYGTLK LHLEMLADQP

Predicted Reactivity: Human: 100%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against PRMT2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PRMT2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 433 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PRMT2 antibody (ABIN2778662)

Zhong, Cao, Liu, Liu, Wang, Liu, Chen, Yang, Zhang, Wu, Ding, Hong, Xiao, Zu, Wen: "Nuclear loss of protein arginine N-methyltransferase 2 in breast carcinoma is associated with tumor grade and overexpression of cyclin D1 protein." in: Oncogene, Vol. 33, Issue 48, pp. 5546-58, (2014) (PubMed).

Meyer, Wolf, Obendorf: "PRMT2, a member of the protein arginine methyltransferase family, is a coactivator of the androgen receptor." in: The Journal of steroid biochemistry and molecular biology, Vol. 107, Issue 1-2, pp. 1-14, (2007) (PubMed).

Target Details for PRMT2

Target: PRMT2 (Protein Arginine Methyltransferase 2 (PRMT2))

Alternative Name: PRMT2 (PRMT2 Products)

Synonyms: PRMT2 antibody, DKFZp459N1919 antibody, HRMT1L1 antibody, Hrmt1l1 antibody, AI504737 antibody, protein arginine methyltransferase 2 antibody, protein arginine methyltransferase 2 L homeolog antibody, protein arginine N-methyltransferase 2 antibody, PRMT2 antibody, Prmt2 antibody, prmt2.L antibody

Background: The protein arginine methyltransferases (PRMTs) include a family of proteins with related putative methyltransferase domains that modify chromatin and regulate cellular transcription. PRMT2 inhibits NF-kappaB-dependent transcription and promotes apoptosis and it exerts this effect by blocking nuclear export of IkappaB-alpha through a leptomycin-sensitive pathway, increasing nuclear IkappaB-alpha and decreasing NF-kappaB DNA binding. PRMT2 also rendered cells susceptible to apoptosis by cytokines or cytotoxic drugs, likely due to its effects on NF-kappaB.
Alias Symbols: HRMT1L1, MGC111373
Protein Interaction Partner: FIP1L1, SPAG8, RIPK2, BAG6, RB1, HNRNPA1, E2F1, UBC, PSMB3, HHV8GK18_gp81, CPSF7, DMRTB1, HNRNPUL1, PRMT2, THRB, PGR, NCOA6, NCOA1, ESR2, RXRA, PRRC2A, ESR1, PPARG, RARA,
Protein Size: 433

Molecular Weight: 49 kDa

Gene ID: 3275

NCBI Accession: NM_001535, NP_001526

UniProt: P55345

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding