GAPDH antibody (N-Term)

Catalog No. ABIN2778645

Product Details anti-GAPDH Antibody

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Dog, Sheep, Horse, Goat, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: IDLNYMVYMF QYDSTHGKFH GTVKAENGKL VINGNPITIF QERDPSKIKW

Predicted Reactivity: Cow: 93%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against GAPDH. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GAPDH

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 335 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GAPDH antibody (ABIN2778645)

Kalinec, Thein, Parsa, Yorgason, Luxford, Urrutia, Kalinec: "Acetaminophen and NAPQI are toxic to auditory cells via oxidative and endoplasmic reticulum stress-dependent pathways." in: Hearing research, Vol. 313, pp. 26-37, (2014) (PubMed).

Fu, Mukhamedova, Ip, DSouza, Henley, DiTommaso, Kesani, Ditiatkovski, Jones, Lane, Jennings, Smyth, Kile, Sviridov: "ABCA12 regulates ABCA1-dependent cholesterol efflux from macrophages and the development of atherosclerosis." in: Cell metabolism, Vol. 18, Issue 2, pp. 225-38, (2013) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH (GAPDH Products)

Synonyms: G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody

Background: GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: G3PD, GAPD, MGC88685
Protein Interaction Partner: FUS, HUWE1, UBC, SUMO2, SUMO3, STAU1, MDM2, GAPDH, ASB15, ASB16, RPA3, RPA2, RPA1, EED, SNCA, rev, HSPA9, HSPA1L, GAS7, TUBA1A, TUFM, RAP1GDS1, PPP5C, PPIB, YWHAG, TUBB, TUBA1C, PRPF40A, UBQLN1, DCPS, MAT2B, ACOT7, METAP2, FERMT2, GBP1, EEF1A1, TYMP, DFFA
Protein Size: 335

Molecular Weight: 36 kDa

Gene ID: 2597

NCBI Accession: NM_002046, NP_002037

UniProt: Q2TSD0