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beta Actin antibody (Middle Region)

Name: beta Actin antibody (Middle Region)
SKU: ABIN2778644
Price: 483.54 USD
Availability: InStock

ACTB Reactivity: Human, Mouse, Rat, Pig, Cow, Sheep, Zebrafish (Danio rerio), Horse, Goat WB, IHC Host: Rabbit Polyclonal unconjugated

WB Suggested Anti-ACTB Antibody Positive Control: Lane 1: 341 µg mouse 3T3 ECM extract + blocking peptide, Lane 2: 041 µg mouse 3T3 ECM extract, Lane 3: 041 µg Echinococcus granulosus extract Primary Antibody Dilution: 1:000Secondary Antibody: Anti-rabbit-HRP Secondry Antibody Dilution: 1:00,000Submitted by: Anonymous (beta Actin antibody (Middle Region))

Host: Rabbit Target Name: ACTB Sample Tissue: HepG2Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1 µg/mL Peptide Concentration: 5. (beta Actin antibody (Middle Region))

Sample Type: NT2 cells Red: Antibody Blue: DAPI Primary Dilution: 1ug/50ul antibody Secondary Antibody: Alexa goat anti-rabbit 594 Image Submitted by: Yuzhi Chen, University of Arkansas for Medical Sciences (beta Actin antibody (Middle Region))

IHC

Sample type: 1-8. (beta Actin antibody (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: Human Adult Placenta Antibody Dilution: 1. (beta Actin antibody (Middle Region))

Host: Rabbit Target Name: ACTBM Sample Type: Fetal Lung lysates Antibody Dilution: 1ug/ml (beta Actin antibody (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: MCF7 Antibody Dilution: 1. (beta Actin antibody (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: HepG2 Whole Cell lysates Antibody Dilution: 1. (beta Actin antibody (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: Human Fetal Lung Antibody Dilution: 1. (beta Actin antibody (Middle Region))

WB Suggested Anti-ACTB Antibody Titration: 0. (beta Actin antibody (Middle Region))

Host: Mouse Target Name: ACTG1 Sample Tissue: Mouse Testis Antibody Dilution: 1ug/ml (beta Actin antibody (Middle Region))

Host: Rabbit Target Name: ACTB Sample Tissue: Mouse Heart Antibody Dilution: 1ug/ml (beta Actin antibody (Middle Region))

Host: Rabbit Target Name: ACTB Sample Type: Hela Antibody Dilution: 1. (beta Actin antibody (Middle Region))

Host: Rabbit Target Name: ACTB Sample Tissue: Human HepG2 Antibody Dilution: 1. (beta Actin antibody (Middle Region))

Sample Type : Human brain stem cells Primary Antibody Dilution : 1:500 Secondary Antibody : Goat anti-rabbit Alexa-Fluor 594 Secondary Antibody Dilution : 1:1000 Color/Signal Descriptions : ACTB: Red DAPI:Blue Gene Name : ACTB Submitted by : Dr. (beta Actin antibody (Middle Region))

IHC

Host: Rabbit Target Name: ACTB Sample Type: HepG2 Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1ug/ml Peptide Concentration: 5. (beta Actin antibody (Middle Region))

IHC Information: Paraffin embedded breast tissue, tested with an antibody dilution of 5 ug/ml. (beta Actin antibody (Middle Region))

IHC

Host: Rabbit Target Name: ACTB Sample Type: Jurkat Antibody Dilution: 1. (beta Actin antibody (Middle Region))

Rabbit Anti-ACTB Antibody Formalin Fixed Paraffin Embedded Tissue: Human Appendix (Colon) Tissue Observed Staining: Cytoplasm Primary Antibody Concentration: 1:100 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0. (beta Actin antibody (Middle Region))

IHC

20 images

(2 references)

Catalog No. ABIN2778644

Datasheet as PDF

Target See all beta Actin (ACTB) Antibodies

beta Actin (ACTB) (Actin, beta (ACTB))
Binding Specificity
39

16

15

6

6

5

5

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
Middle Region
Reactivity
175

125

107

56

42

42

36

25

23

22

17

17

12

10

9

6

6

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1
Human, Mouse, Rat, Pig, Cow, Sheep, Zebrafish (Danio rerio), Horse, Goat
Host
112

78

5

1

1
Rabbit
Compatible Secondaries
Clonality
110

85
Polyclonal
Conjugate
123

12

12

10

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This beta Actin antibody is un-conjugated
Application
164

70

50

46

35

31

26

22

13

13

8

6

6

5

5

2

1

1
Western Blotting (WB), Immunohistochemistry (IHC)

Sequence

TMKIKIIAPP ERKYSVWIGG SILASLSTFQ QMWISKQEYD ESGPSIVHRK

Predicted Reactivity

Cow: 100%, Goat: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics

This is a rabbit polyclonal antibody against ACTB. It was validated on Western Blot.

Purification

Affinity Purified

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of Human ACTB

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anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human WB, IF, IP Host: Mouse Monoclonal unconjugated

anti-Actin, beta (ACTB) (AA 359-368) antibody
ACTB Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 2-16) antibody
ACTB Reactivity: Mouse, Rat, Chicken, Zebrafish (Danio rerio) WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human, Mouse, Rat WB, IF, IHC (p), FACS, DNA Mic Host: Mouse Monoclonal 137CT26-1-1 unconjugated

anti-Actin, beta (ACTB) (AA 1-25) antibody
ACTB Reactivity: Human, Mouse, Rat, Pig, Cow WB, IHC (p) Host: Mouse Monoclonal 1A2 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 3G4-F9 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
KO Validated ACTB Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human, Mouse, Chicken, Rabbit, Pig, Cow, Hamster, Goat, Fish WB, ELISA Host: Mouse Monoclonal 2D1D10 unconjugated

Application Notes

Optimal working dilutions should be determined experimentally by the investigator.

Comment

Antigen size: 375 AA

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and may contain up to 2 % sucrose.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freeze-thaw cycles.

Storage

-20 °C

Storage Comment

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Phungphong, Kijtawornrat, Wattanapermpool, Bupha-Intr: "Regular exercise modulates cardiac mast cell activation in ovariectomized rats." in: The journal of physiological sciences : JPS, Vol. 66, Issue 2, pp. 165-73, (2016) (PubMed).

Rinne, Clements, Lamme, Duijf, Bolat, Meijer, Scheffer, Rosser, Tan, McGrath, Schalkwijk, Brunner, Zhou, van Bokhoven: "A novel translation re-initiation mechanism for the p63 gene revealed by amino-terminal truncating mutations in Rapp-Hodgkin/Hay-Wells-like syndromes." in: Human molecular genetics, Vol. 17, Issue 13, pp. 1968-77, (2008) (PubMed).
Target

beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name

ACTB (ACTB Products)

Background

This protein is one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Alias Symbols: BRWS1, PS1TP5BP1
Protein Interaction Partner: FUS, DSTN, CAP2, CFL2, CFL1, ACTG1, ACTB, PA2G4, EHHADH, WDYHV1, HDAC6, ISG15, TP53, CEP250, TUBGCP2, TUBGCP3, VCP, UBC, TUBG1, SUMO2, LGALS3BP, AMOT, NEDD1, HAUS1, IVNS1ABP, STAU1, MDM2, CORO7, LGR4, ASB12, ASB9, ZBTB1, ERG, RPA3, RPA2, RPA1, CDKN2A, PAR
Protein Size: 375

Molecular Weight

42 kDa

Gene ID

60

NCBI Accession

NM_001101, NP_001092

UniProt

P60709

Pathways

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction