INSIG1 antibody (Middle Region)

Catalog No. ABIN2778638

Product Details anti-INSIG1 Antibody

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Horse, Rabbit, Cow, Goat, Guinea Pig, Sheep, Dog, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This INSIG1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: WWVPPCCGTA SAVIGLLYPC IDRHLGEPHK FKREWSSVMR CVAVFVGINH

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 87%

Characteristics: This is a rabbit polyclonal antibody against INSIG1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human INSIG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 225 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for INSIG1

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Alternative Name: INSIG1 (INSIG1 Products)

Synonyms: cl-6 antibody, cl6 antibody, CL-6 antibody, CL6 antibody, fb55a03 antibody, fi35b10 antibody, wu:fb55a03 antibody, wu:fi35b10 antibody, zgc:55439 antibody, 1810013C12Rik antibody, Insig-1 antibody, INSIG-1 antibody, insulin induced gene 1 antibody, insulin induced gene 1 L homeolog antibody, INSIG1 antibody, insig1 antibody, insig1.L antibody, Insig1 antibody

Background: Oxysterols regulate cholesterol homeostasis through the liver X receptor (LXR)- and sterol regulatory element-binding protein (SREBP)-mediated signaling pathways. This gene is an insulin-induced gene. INSIG1 is an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. INSIG1 binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins.The protein encoded by this gene is highly similar to the protein product encoded by gene INSIG1. Both INSIG1 protein and this protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: MGC26273, INSIG1
Protein Interaction Partner: RNF5, HMGCR, SCAP, SREBF1, RNF139, UBQLN1, UBQLN2, FAF2, ADRM1, PSMD14, BAG6, VCP, UBC, RPN1, PSMD13, PSMD12, PSMD11, PSMD4, PSMC6, PSMC5, PSMC3, PSMC2, PSMB3, PSMB1, PSMA7, PSMA2, LGALS7, JUP, INSIG1, HSPA8, HSPA6, HSPA5, HSPA1A, ENO1, DSP, AMFR, SREBF2,
Protein Size: 225

Molecular Weight: 25 kDa

Gene ID: 51141

NCBI Accession: NM_016133, NP_057217

UniProt: Q9Y5U4

Pathways: ER-Nucleus Signaling