RNF34 antibody (Middle Region)

Catalog No. ABIN2778633

Product Details anti-RNF34 Antibody

Target: RNF34 (Ring Finger Protein 34 (RNF34))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Cow, Dog, Rabbit, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF34 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LVDLVLCHHG LGSEDDMDTS SLNSSRSQTS SFFTRSFFSN YTAPSATMSS

Predicted Reactivity: Cow: 87%, Dog: 93%, Guinea Pig: 80%, Horse: 93%, Human: 100%, Rabbit: 80%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against RNF34. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RNF34

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 372 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RNF34

Target: RNF34 (Ring Finger Protein 34 (RNF34))

Alternative Name: RNF34 (RNF34 Products)

Background: RNF34 contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis.The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Alias Symbols: FLJ21786, RFI, RIF, RIFF, hRFI, CARP1, CARP-1
Protein Interaction Partner: APPBP2, NOD1, APP, PRKACB, PHF7, TRIM23, UBE2D2, CASP10, CASP8, FZR1, ANAPC2, CDC20, SH3KBP1, PPARGC1A, TP53, UBE2F, UBE2H, DNAJA3,
Protein Size: 372

Molecular Weight: 42 kDa

Gene ID: 80196

NCBI Accession: NM_025126, NP_079402

UniProt: Q969K3