AFF3 antibody (Middle Region)

Catalog No. ABIN2778345

Product Details anti-AFF3 Antibody

Target: AFF3 (AF4/FMR2 Family, Member 3 (AFF3))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFF3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: APATKDSESA PPSHTSDTPA EKALPKSKRK RKCDNEDDYR EIKKSQGEKD

Predicted Reactivity: Dog: 86%, Human: 100%, Mouse: 93%, Pig: 86%, Rabbit: 77%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against AFF3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human AFF3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1226 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-AFF3 antibody (ABIN2778345)

To, Faseruk, Gokgoz, Pinnaduwage, Done, Andrulis: "LAF-4 is aberrantly expressed in human breast cancer." in: International journal of cancer. Journal international du cancer, Vol. 115, Issue 4, pp. 568-74, (2005) (PubMed).

Target Details for AFF3

Target: AFF3 (AF4/FMR2 Family, Member 3 (AFF3))

Alternative Name: AFF3 (AFF3 Products)

Synonyms: id:ibd5053 antibody, LAF4 antibody, MLLT2-like antibody, 3222402O04Rik antibody, A730046J16 antibody, LAF-4 antibody, Laf4 antibody, AF4/FMR2 family member 3 antibody, AF4/FMR2 family, member 3 antibody, AFF3 antibody, aff3 antibody, Aff3 antibody

Background: AFF3 is a tissue-restricted nuclear transcriptional activator that is preferentially expressed in lymphoid tissue. Isolation of this protein initially defined a highly conserved LAF4/MLLT2 gene family of nuclear transcription factors that may function in lymphoid development and oncogenesis. In some ALL patients, this gene has been found fused to the gene for MLL.This gene encodes a tissue-restricted nuclear transcriptional activator that is preferentially expressed in lymphoid tissue. Isolation of this protein initially defined a highly conserved LAF4/MLLT2 gene family of nuclear transcription factors that may function in lymphoid development and oncogenesis. In some ALL patients, this gene has been found fused to the gene for MLL. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.
Alias Symbols: LAF4, MLLT2-like
Protein Interaction Partner: CDK9, MLLT3,
Protein Size: 1226

Molecular Weight: 133 kDa

Gene ID: 3899

NCBI Accession: NM_002285, NP_002276

UniProt: P51826